Product nameAnti-Protein Kinase D2 antibody
See all Protein Kinase D2 primary antibodies
DescriptionRabbit polyclonal to Protein Kinase D2
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Human
Human protein kinase D2.
This antibody recognizes human protein kinase D2 (PKD2), a serine threonine protein kinase. PKD2 is widely expressed in human and murine tissues and exhibits strong homology to the serine threonine protein kinases PKD/PKCmicro and PKCnu. In contrast, it has a low degree of sequence homology to other members of the PKC family. PKD2 is activated by phorbol esters and a variety of growth factors.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesThis antibody recognizes human protein kinase D2 (PKD2), a serine threonine protein kinase. PKD2 is widely expressed in human and murine tissues and exhibits strong homology to the serine threonine protein kinases PKD/PKCmicro and PKCnu. In contrast, it has a low degree of sequence homology to other members of the PKC family. PKD2 is activated by phorbol esters and a variety of growth factors.
Our Abpromise guarantee covers the use of ab7281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 105 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
FunctionConverts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
modificationsPhosphorylation of Ser-876 correlates with the activation status of the kinase.
Ser-706 is probably phosphorylated by PKC.
Cellular localizationCytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
- Information by UniProt
- DKFZp586E0820 antibody
- HSPC187 antibody
- KPCD2_HUMAN antibody
Anti-Protein Kinase D2 antibody (ab7281) at 1/500 dilution + whole cell lysate prepared from murine NIH3T3 cells at 25 µg
Donkey anti-rabbit HRP at 1/5000 dilution
Developed using the ECL technique.
Exposure time: 15 minutes
ab7281 staining Protein Kinase D2 in human BON cells (derived from a human pancreatic carcinoid tumor) by Immunocytochemistry/ Immunofluorescence.
BON cells were grown on glass coverslips in 24-well plates. 2 or 3 days after seeding or transfection, cells were treated with vehicle (Me2SO) or PMA (100 nm) for 30 minutes. After treatment, cells were fixed with 4% paraformaldehyde for 20 minutes at 37 °C. After three washes with PBS, the cells were permeabilized with 0.3% Triton X-100 for 15 minutes at 37 °C and blocked with 1% bovine serum albumin/PBS for 10 minutes. The cells were incubated with the primary antibody diluted with 1% bovine serum albumin/PBS for 1 hour at room temperature. Cells were washed three times with PBS and incubated with Alexa-conjugated secondary antibody diluted by 1/500 in 1% bovine serum albumin/PBS.
Double staining was performed using antibodies ab7281 and an anti-NT. Co-localization is shown by arrows in the merged images.
ICC/IF image of ab7281 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7281, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protein Kinase D2 was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to Protein Kinase D2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7281.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 101KDa: Protein Kinase D2
This product has been referenced in:
- Xiao Y et al. Deficiency of PRKD2 triggers hyperinsulinemia and metabolic disorders. Nat Commun 9:2015 (2018). Read more (PubMed: 29789568) »
- Arun SN et al. Ultraviolet B irradiation and activation of protein kinase D in primary mouse epidermal keratinocytes. Oncogene : (2010). WB ; Mouse . Read more (PubMed: 21132013) »