Overview

  • Product name
    Anti-Protein Kinase D2 (phospho S876) antibody [EP1496Y]
    See all Protein Kinase D2 primary antibodies
  • Description
    Rabbit monoclonal [EP1496Y] to Protein Kinase D2 (phospho S876)
  • Host species
    Rabbit
  • Specificity
    This antibody detects Protein Kinase D2 when phosphorylated at Ser876.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding Serine 876 of Human Protein Kinase D2.

  • Positive control
    • HeLa cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    EP1496Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: 1/40.
    WB: 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).

    Is unsuitable for Flow Cyt, IHC or ICC/IF.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
      Contains 1 PH domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylation of Ser-876 correlates with the activation status of the kinase.
      Ser-706 is probably phosphorylated by PKC.
    • Cellular localization
      Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp586E0820 antibody
      • HSPC187 antibody
      • KPCD2_HUMAN antibody
      • nPKC D2 antibody
      • nPKC-D2 antibody
      • PKD2 antibody
      • PRKD 2 antibody
      • Prkd2 antibody
      • Protein kinase D2 antibody
      • Serine/threonine protein kinase D2 antibody
      • Serine/threonine-protein kinase D2 antibody
      see all

    Images

    • All lanes : Anti-Protein Kinase D2 (phospho S876) antibody [EP1496Y] (ab51251) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with TPA

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 97 kDa
      Observed band size: 105 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:
    • Bernhart E  et al. Protein kinase D2 regulates migration and invasion of U87MG glioblastoma cells in vitro. Exp Cell Res 319:2037-48 (2013). Read more (PubMed: 23562655) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary murine microglia)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (7%)
    Loading amount
    100 µg
    Treatment
    Lysophosphatidic acid (LPA) 1µM
    Specification
    Primary murine microglia
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Joanna Plastira

    Verified customer

    Submitted Oct 12 2018

    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Left ventricle)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Left ventricle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Christopher Trummer

    Verified customer

    Submitted Oct 04 2018

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Left Ventricle)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Left Ventricle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Christopher Trummer

    Verified customer

    Submitted Oct 04 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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