Product nameAnti-Protein Kinase D2 (phospho S876) antibody [EP1496Y]
See all Protein Kinase D2 primary antibodies
DescriptionRabbit monoclonal [EP1496Y] to Protein Kinase D2 (phospho S876)
SpecificityThis antibody detects Protein Kinase D2 when phosphorylated at Ser876.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
Synthetic phospho-peptide corresponding to residues surrounding Serine 876 of Human Protein Kinase D2.
- HeLa cell lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab51251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).
Is unsuitable for Flow Cyt, IHC or ICC/IF.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionConverts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
modificationsPhosphorylation of Ser-876 correlates with the activation status of the kinase.
Ser-706 is probably phosphorylated by PKC.
Cellular localizationCytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
- Information by UniProt
- DKFZp586E0820 antibody
- HSPC187 antibody
- KPCD2_HUMAN antibody
All lanes : Anti-Protein Kinase D2 (phospho S876) antibody [EP1496Y] (ab51251) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with TPA
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?