• Product name

    Anti-Protein L antibody (Biotin)
    See all Protein L primary antibodies
  • Description

    Chicken polyclonal to Protein L (Biotin)
  • Host species

  • Conjugation

  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with bacteria.
  • Immunogen

    Purified recombinant Protein L.


Associated products


Our Abpromise guarantee covers the use of ab63505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.
ELISA Use at an assay dependent dilution.


  • Relevance

    Protein L is a 36,000 dalton immunoglobulin-binding protein isolated from the bacteria Peptostreptococcus magnus. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies through light chain interactions. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L. Despite this wide binding range, Protein L is not a universal antibody-binding protein. Protein L binding is restricted to those antibodies that contain kappa light chains and it is only effective in binding certain subtypes of kappa light chains - about 65% of human immunoglobulins carry kappa light chains. Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLkappa-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobulins, which are often present in the media as a serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.
  • Cellular localization

    Cell surface
  • Alternative names

    • ProteinL antibody
    • RPL antibody


ab63505 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for your enquiry.

Currently, we have 4 antibodies against Protein L but none of them have been tested in immunoprecipitation:

ab63503, ab63504, ab63505, ab63506

If your customer would like to test any of these antibodies in IP and get a Discount code from us, please do let me know. The Terms and Conditions of this testing offer can be found at: https://www.abcam.com/collaborationdiscount.

If you need anything further or any help then please let me know.

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