Overview

  • Product name

    Anti-Protein L antibody (HRP)
    See all Protein L primary antibodies
  • Description

    Chicken polyclonal to Protein L (HRP)
  • Host species

    Chicken
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with bacteria.
  • Immunogen

    Purified recombinant Protein L.

Properties

Applications

Our Abpromise guarantee covers the use of ab63506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    Protein L is a 36,000 dalton immunoglobulin-binding protein isolated from the bacteria Peptostreptococcus magnus. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies through light chain interactions. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L. Despite this wide binding range, Protein L is not a universal antibody-binding protein. Protein L binding is restricted to those antibodies that contain kappa light chains and it is only effective in binding certain subtypes of kappa light chains - about 65% of human immunoglobulins carry kappa light chains. Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLkappa-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobulins, which are often present in the media as a serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.
  • Cellular localization

    Cell surface
  • Alternative names

    • ProteinL antibody
    • RPL antibody

References

ab63506 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your response.

A lysate ofPeptostreptococcus magnus,purified Protein L protein or recombinant Protein Lwould all be ideal positive controls for use withab63506.

Fora positive control to use with our anti GBA antibody, ab55080. We have data on our website which shows the antibody to work withMCF-7 cell lysate and withHN10 cells. I would recommend one of those lysates for a positive control.

I would like to encourage you to take advantage of our Abreview program. It takes just a few minutes to leave a review and you can collect Abpoints which you may redeem for Abcam products or Amazon gift cards while at the same time sharing information about the product with your colleagues worldwide. More information may be at:

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I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

Thank you for contacting us.

When choosing a loading control it is best practice to choose housekeeping genes which will be present in your sample type. For example, in whole cell preparations beta Actin or GAPDH will be appropriate while in nuclear or mitochondrial fractions use Lamin B1 or COXIV respectively.

These controls are excellent when you sample is eukaryotic. However, as Protein L will be from bacteria these housekeeping proteins may not be present. I have found however that GAPDH may be found in E.coli.

I would recommend therefore that if your sample types are whole cell/membrane or cytoplasmic and are either bacterial (in the case of the Protein L antibody ab63506) or eukaryotic (as for use with anti GBA antibody ab55080) that you use our anti-GAPDH antibodies.

We do have one anti-GAPDH product which is guaranteed to work with E.coli, Ab85760. Further information about this product may be found at the following page:

https://www.abcam.com/GAPDH-antibody-HRP-ab85760.html


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry.

Currently, we have 4 antibodies against Protein L but none of them have been tested in immunoprecipitation:

ab63503, ab63504, ab63505, ab63506

If your customer would like to test any of these antibodies in IP and get a Discount code from us, please do let me know. The Terms and Conditions of this testing offer can be found at: https://www.abcam.com/collaborationdiscount.

If you need anything further or any help then please let me know.

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