Protein L Sepharose® (ab193261)
Overview
-
Product name
Protein L Sepharose® -
Product overview
Contents:
Supplied as a 50% slurry in 20% Ethanol, >5 mg Protein L/mL of Sepharose® beads.
Features:
Binding capacity of human IgG is greater than 10 mg/mL of gel; high flow rate; low falling off of rProtein L; pH stability 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
- Purification of monoclonal and polyclonal antibodies.
- Immunoprecipitation.
Sepharose is a registered trademark of GE Healthcare
-
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 6531 Protein L Sepharose. 6531-25 is the same size as the 25 ml size of ab193261.
Protein L Sepharose® is prepared by covalently coupling recombinant Protein L (which contains five Ig light chain binding domains) to 6% cross-linked Sepharose® beads. The coupling technique is optimized to give high binding capacity. The capacity of IgG binding is generally greater than 10 mg of human IgG per mL of wet gel. Protein L binds to a wider range of antibody classes than Protein A or Protein G, including human, mouse and rat IgM, IgA, IgE, and IgD, as well as Fab and single chain variable fragments (scFv). Protein L binds only binds to antibodies that contain Κ light chains, which are found in most human and mouse antibodies.
-
Tested applications
Suitable for: Purification, IPmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 5 ml 100 ml 25 ml Protein L Sepharose® 1 x 1ml 1 x 5ml 1 x 100ml 1 x 25ml -
Research areas
Associated products
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Purification |
Use at an assay dependent concentration.
Purification of monoclonal and polyclonal antibodies. |
|
IP |
Use at an assay dependent concentration.
|
Notes |
---|
Purification
Use at an assay dependent concentration. Purification of monoclonal and polyclonal antibodies. |
IP
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab193261 has not yet been referenced specifically in any publications.