Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab16369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa.
IP Use at an assay dependent dilution.

Target

  • Function
    Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Cellular localization
    Cytoplasm. Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC3672 antibody
    • PP 1B antibody
    • PP-1B antibody
    • PP1B antibody
    • PP1B_HUMAN antibody
    • PP1beta antibody
    • PPP1CB antibody
    • PPP1CD antibody
    • Protein phosphatase 1 beta antibody
    • Protein phosphatase 1 catalytic subunit beta isoform antibody
    • Protein phosphatase 1 delta antibody
    • Protein phosphatase 1, catalytic subunit, beta isozyme antibody
    • Protein phosphatase 1, catalytic subunit, delta isoform antibody
    • Serine threonine protein phosphatase PP1 beta catalytic subunit antibody
    • Serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody
    see all

References

This product has been referenced in:
  • Miyazaki Y  et al. Heart failure-inducible gene therapy targeting protein phosphatase 1 prevents progressive left ventricular remodeling. PLoS One 7:e35875 (2012). WB . Read more (PubMed: 22558250) »
  • Barber MA  et al. The guanine-nucleotide-exchange factor P-Rex1 is activated by protein phosphatase 1a. Biochem J 443:173-83 (2012). WB . Read more (PubMed: 22242915) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your response. I would like to confirm that every batch has been tested QC analysis and have just placed a new order for you for one vial of ab16369 as a free of charge replacement. Your new order number is 136944 and as you should be able to receive the new vial very shortly. Thank you for your patience in advance and please do get back to me and let me know how you are getting on with the second vial.

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Answer

Thank you for your email. As the antibody is currently out of stock, it will take approximately 3-4 weeks for a new batch to come into stock. I unfortunately can't hasten the process. Please contact us again if you have any additional questions.

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Answer

Thank you for your enquiry. I checked this antibody, and the sequence of the peptide used to generate ab16369 was indeed PRTANPPKKR, corresponding to amino acids 318-327 of Human PPP1B. However, the database links were wrong and I have updated them. Thank you for pointing this out! We have not yet received feedback from customers who have used this antibody. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abpoints which can be redeemed on a number of rewards (a further 100 Abpoints will be offered for an image). Thanks again, and please contact us again if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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