Product nameAnti-Protein phosphatase 1 gamma 2 antibody [EPR8934]
See all Protein phosphatase 1 gamma 2 primary antibodies
DescriptionRabbit monoclonal [EPR8934] to Protein phosphatase 1 gamma 2
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Protein phosphatase 1 gamma 2 aa 250-350 (C terminal). The exact sequence is proprietary.
- 293T, HeLa, SKBR3 and T47D cell lysates.
Storage instructionsShipped at 4°C. Store at -20ºC.
Dissociation constant (KD)KD = 4.20 x 10 -11 M Learn more about KD
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 39 kDa.|
FunctionProtein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Dephosphorylates RPS6KB1. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
modificationsPhosphorylated by NEK2.
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus speckle. Chromosome > centromere > kinetochore. Cleavage furrow. Midbody. Mitochondrion. Colocalizes with SPZ1 in the nucleus (By similarity). Colocalizes with URI1 at mitochondrion. Rapidly exchanges between the nucleolar, nucleoplasmic and cytoplasmic compartments. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleolus to nuclear speckles. Shows a dynamic targeting to specific sites throughout the cell cycle. Highly concentrated in nucleoli of interphase cells and localizes at kinetochores early in mitosis. Relocalization to chromosome-containing regions occurs at the transition from early to late anaphase. Also accumulates at the cleavage furrow and midbody by telophase.
- Information by UniProt
- ppp1cc-a antibody
- PP 1G antibody
- PP-1G antibody
All lanes : Anti-Protein phosphatase 1 gamma 2 antibody [EPR8934] (ab134947) at 1/1000 dilution
Lane 1 : T47D cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SKBR3 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Cai J et al. Simultaneous overactivation of Wnt/ß-catenin and TGFß signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC. Nat Commun 8:15870 (2017). Read more (PubMed: 28627514) »
- Garbacz WG et al. Hepatic Overexpression of CD36 Improves Glycogen Homeostasis and Attenuates High-Fat Diet-Induced Hepatic Steatosis and Insulin Resistance. Mol Cell Biol 36:2715-2727 (2016). Read more (PubMed: 27528620) »