Product nameAnti-Protein phosphatase 1 inhibitor subunit 2 antibody
See all Protein phosphatase 1 inhibitor subunit 2 primary antibodies
DescriptionRabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2
Specificityab73948 detects endogenous levels of total Protein phosphatase 1 inhibitor subunit 2 protein.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic non-phosphopeptide derived from human Protein phosphatase 1 inhibitor subunit 2 around the phosphorylation site of serine 120 and serine 121 (Q-E-SP-SP-G-E).
- Human breast carcinoma tissue. Extracts from Jurkat cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab73948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionInhibitor of protein-phosphatase 1.
Sequence similaritiesBelongs to the protein phosphatase inhibitor 2 family.
modificationsPhosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
- Information by UniProt
- IPP 2 antibody
- IPP-2 antibody
- IPP2 antibody
All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 antibody (ab73948) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab73948, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
ab73948 has not yet been referenced specifically in any publications.