Anti-Protein phosphatase 1 inhibitor subunit 2 antibody (ab73948)
Key features and details
- Rabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Protein phosphatase 1 inhibitor subunit 2 antibody
See all Protein phosphatase 1 inhibitor subunit 2 primary antibodies -
Description
Rabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic non-phosphopeptide derived from human Protein phosphatase 1 inhibitor subunit 2 around the phosphorylation site of serine 120 and serine 121 (Q-E-SP-SP-G-E).
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Positive control
- Human breast carcinoma tissue. Extracts from Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa). |
IHC-P
1/50 - 1/100. |
Target
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Function
Inhibitor of protein-phosphatase 1. -
Sequence similarities
Belongs to the protein phosphatase inhibitor 2 family. -
Post-translational
modificationsPhosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex. - Information by UniProt
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Database links
- Entrez Gene: 5504 Human
- Entrez Gene: 66849 Mouse
- Entrez Gene: 192361 Rat
- Omim: 601792 Human
- SwissProt: P41236 Human
- SwissProt: Q9DCL8 Mouse
- SwissProt: P50411 Rat
- Unigene: 535731 Human
see all -
Alternative names
- IPP 2 antibody
- IPP-2 antibody
- IPP2 antibody
see all
Images
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All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 antibody (ab73948) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab73948, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab73948 has not yet been referenced specifically in any publications.