Overview

  • Product name

    Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody
    See all Protein phosphatase 1 inhibitor subunit 2 primary antibodies
  • Description

    Rabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2 (phospho T72)
  • Host species

    Rabbit
  • Specificity

    ab27850 recognises Inhibitor-2.
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide (Human) - derived from the region that contains threonine 72.

  • Positive control

    • ICC/IF: A431 cells treated with Nocodazole and HeLa cells; WB: HCT116, HT 29, HEL 92.1.7 and HeLa whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 0.1% BSA

    1.0 mg/mL BSA (IgG, protease free), Dulbecco's PBS [without magnesium and calcium ions]
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Inhibitor-2. The final product is generated by affinity chromatography using an Inhibitor-2 -derived peptide that is phosphorylated at threonine 72.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 23 kDa.
ICC 1/100.

Target

  • Function

    Inhibitor of protein-phosphatase 1.
  • Sequence similarities

    Belongs to the protein phosphatase inhibitor 2 family.
  • Post-translational
    modifications

    Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • IPP 2 antibody
    • IPP-2 antibody
    • IPP2 antibody
    • IPP2_HUMAN antibody
    • MGC87148 antibody
    • Phosphatase inhibitor 2 antibody
    • Phosphoprotein phosphatase antibody
    • Ppp1r2 antibody
    • PPP1R2 protein antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 2 antibody
    • Protein phosphatase 1 regulatory subunit 2 antibody
    • Protein phosphatase inhibitor 2 antibody
    see all

Images

  • All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution

    Lane 1 : HCT 116 whole cell lysate
    Lane 2 : HT 29 whole cell lysate
    Lane 3 : Hel 92.1.7 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Secondary Antibody at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/Immunofluorescent analysis of A431 cells treated with Nocodazole for 24 hours, staining Protein phosphatase 1 inhibitor subunit 2 at 1/250 dilution, followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate  at 1/2000 dilution (green). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X 100 for ten minutes, and blocked with 1% BSA for one hour at room temperature. Nuclei were counterstained with DAPI (blue). F-actin was stained with Rhodamine Phalloidin (red).

    Negative controls are untreated A431 cells (e) and a secondary only antibody control (f).

     

  • Immunocyutochemistry/Immunofluroescence analysis of HeLa cells staining Protein phosphatase 1 inhibitor subunit 2 with ab27850 (green). Cells were grown on a chamber slide and fixed in cold 10% trichloroacetic acid for 10 minutes, rinsed in TBS and permeabilized with 0.1% Triton-X-100 for 15 minutes. 

References

ab27850 has not yet been referenced specifically in any publications.

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