Product nameAnti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody
See all Protein phosphatase 1 inhibitor subunit 2 primary antibodies
DescriptionRabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2 (phospho T72)
Specificityab27850 recognises Inhibitor-2.
Tested applicationsSuitable for: WB, ICCmore details
Species reactivityReacts with: Human
Synthetic phosphopeptide (Human) - derived from the region that contains threonine 72.
- ICC/IF: A431 cells treated with Nocodazole and HeLa cells; WB: HCT116, HT 29, HEL 92.1.7 and HeLa whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA
1.0 mg/mL BSA (IgG, protease free), Dulbecco's PBS [without magnesium and calcium ions]
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Inhibitor-2. The final product is generated by affinity chromatography using an Inhibitor-2 -derived peptide that is phosphorylated at threonine 72.
Our Abpromise guarantee covers the use of ab27850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 23 kDa.|
FunctionInhibitor of protein-phosphatase 1.
Sequence similaritiesBelongs to the protein phosphatase inhibitor 2 family.
modificationsPhosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
- Information by UniProt
- IPP 2 antibody
- IPP-2 antibody
- IPP2 antibody
All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution
Lane 1 : HCT 116 whole cell lysate
Lane 2 : HT 29 whole cell lysate
Lane 3 : Hel 92.1.7 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : Goat Anti-Rabbit IgG Secondary Antibody at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Immunocytochemistry/Immunofluorescent analysis of A431 cells treated with Nocodazole for 24 hours, staining Protein phosphatase 1 inhibitor subunit 2 at 1/250 dilution, followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X 100 for ten minutes, and blocked with 1% BSA for one hour at room temperature. Nuclei were counterstained with DAPI (blue). F-actin was stained with Rhodamine Phalloidin (red).
Negative controls are untreated A431 cells (e) and a secondary only antibody control (f).
Immunocyutochemistry/Immunofluroescence analysis of HeLa cells staining Protein phosphatase 1 inhibitor subunit 2 with ab27850 (green). Cells were grown on a chamber slide and fixed in cold 10% trichloroacetic acid for 10 minutes, rinsed in TBS and permeabilized with 0.1% Triton-X-100 for 15 minutes.
ab27850 has not yet been referenced specifically in any publications.