Anti-Protein S antibody (ab97387)
Key features and details
- Rabbit polyclonal to Protein S
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Protein S antibody
See all Protein S primary antibodies -
Description
Rabbit polyclonal to Protein S -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey
-
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 209 and 447 of Human protein S (NP_000304).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 75 kDa.
|
| ICC/IF |
1/100 - 1/200.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 75 kDa. |
|
ICC/IF
1/100 - 1/200. |
Target
-
Function
Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in PROS1 are the cause of protein S deficiency (PROS1D) [MIM:612336]; also known as thrombophilia due to protein S deficiency. PROS1D is a cause of hereditary thrombophilia, a hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Based on the plasma levels of total and free PROS1 antigen as well as the serine protease-activated protein C cofactor activity, three types of PROS1D have been described: type I, characterized by reduced total and free PROS1 antigen levels together with reduced anticoagulant activity; type III, in which only free PROS1 antigen and PROS1 activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free PROS1 antigen, but low cofactor activity. -
Sequence similarities
Contains 4 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 laminin G-like domains. -
Post-translational
modificationsThe iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 5627 Human
- Omim: 176880 Human
- SwissProt: P07225 Human
- Unigene: 64016 Human
-
Alternative names
- Preproprotein S antibody
- Propiece of latent protein S antibody
- PROS 1 antibody
see all
Images
-
Western blot - Anti-Protein S antibody (ab97387)Anti-Protein S antibody (ab97387) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 75 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Protein S antibody (ab97387)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97387 at 1/200 dilution. Lower image merged with DNA probe.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab97387 has been referenced in 1 publication.
- Abud EM et al. iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases. Neuron 94:278-293.e9 (2017). PubMed: 28426964