Recombinant
RabMAb

Recombinant Anti-Protein S antibody [EPSISR9] (ab133730)

Submit a review Q&A (1)

Overview

  • Product name

    Anti-Protein S antibody [EPSISR9]
    See all Protein S primary antibodies

  • Description

    Rabbit monoclonal [EPSISR9] to Protein S

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Protein S aa 550-650. The exact sequence is proprietary.

  • Positive control

    • Human plasma lysate

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 75 kDa.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.

    • Tissue specificity

      Plasma.

    • Involvement in disease

      Defects in PROS1 are the cause of protein S deficiency (PROS1D) [MIM:612336]; also known as thrombophilia due to protein S deficiency. PROS1D is a cause of hereditary thrombophilia, a hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Based on the plasma levels of total and free PROS1 antigen as well as the serine protease-activated protein C cofactor activity, three types of PROS1D have been described: type I, characterized by reduced total and free PROS1 antigen levels together with reduced anticoagulant activity; type III, in which only free PROS1 antigen and PROS1 activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free PROS1 antigen, but low cofactor activity.

    • Sequence similarities

      Contains 4 EGF-like domains.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 2 laminin G-like domains.

    • Post-translational
      modifications

      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Preproprotein S antibody
        • Propiece of latent protein S antibody
        • PROS 1 antibody
        • PROS antibody
        • PROS_HUMAN antibody
        • proS1 antibody
        • Protein S alpha antibody
        • Protein Sa antibody
        • PS 21 antibody
        • PS 22 antibody
        • PS 23 antibody
        • PS 24 antibody
        • PS 25 antibody
        • PS 26 antibody
        • PS21 antibody
        • PS22 antibody
        • PS23 antibody
        • PS24 antibody
        • PS25 antibody
        • PS26 antibody
        • PSA antibody
        • THPH5 antibody
        • THPH6 antibody
        • Vitamin K dependent protein S antibody
        • Vitamin K-dependent plasma protein S antibody
        • Vitamin K-dependent protein S antibody
        see all

      Images

      • Western blot - Anti-Protein S antibody [EPSISR9] (ab133730)
        Western blot - Anti-Protein S antibody [EPSISR9] (ab133730)
        Anti-Protein S antibody [EPSISR9] (ab133730) at 1/1000 dilution + Human plasma lysate at 10 µg

        Secondary
        HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

        Predicted band size: 75 kDa

      References

      ab133730 has not yet been referenced specifically in any publications.

      Publishing research using ab133730? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Inquiry: Dear abcam, I was supplied 100uL, but what is the concentration of the mAb in this lot? Best regards,

      Read More
      Answer

      Thank you for your enquiry.

      I can confirm that ab133730antibody is sold astissue culture supernatant. Unpurified antibodies, such as those sold as tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

      I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

      I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com