Key features and details
- Rabbit polyclonal to Protein Z
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Protein Z antibody
See all Protein Z primary antibodies
DescriptionRabbit polyclonal to Protein Z
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human Protein Z aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in HepG2 whole cell lysate as well as the following tissue lysates: Human Heart; Human Spleen; Human Bone Tumour; Human Bone Marrow. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: DU145.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).|
FunctionAppears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
modificationsThe iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
- Information by UniProt
- Protein Z, vitamin K dependent plasma glycoprotein antibody
- PROZ antibody
- PROZ_HUMAN antibody
All lanes : Anti-Protein Z antibody (ab85317) at 1 µg/ml (Milk Blocking - 5%)
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human bone tumor tissue lysate - total protein (ab29359)
Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa, 55 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 42 kDa could potentially be a cleaved form of Protein Z due to the presemce of a 23 amino acid signal peptide.
ICC/IF image of ab85317 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85317 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab85317 has not yet been referenced specifically in any publications.