Proteinase K Antigen Retrieval Solution (ab64220)
Overview
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Product name
Proteinase K Antigen Retrieval Solution -
Tested applications
Suitable for: IHC-P, In situ hybridizationmore details -
General notes
ab64220 is a single-component, ready-to-use proteinase K solution for use in protease-induced antigen retrieval during IHC (PIER) and pre-treatment for in situ hybridization.
Staining Protocol:
- IHC procedure: Incubate tissue section for 5 to 7 minutes at room temperature; Rinse and proceed with immunostaining.
- General ISH procedure: Incubate tissue section for 15 minutes at 37oC; Rinse and proceed with hybridization.
Other products for IHC
Other enzymes for antigen retrieval include: Pepsin Solution ab64201 and Trypsin Antigen Retrieval Kit ab259256.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Properties
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Form
Liquid -
Storage instructions
Store at -20°C. Stable for 12 months at -20°C -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: Tris buffer, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Relevance
Proteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name. Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes. -
Alternative names
- Endopeptidase K
- Tritirachium alkaline proteinase
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| In situ hybridization |
Use at an assay dependent dilution.
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| Notes |
|---|
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IHC-P
Use at an assay dependent dilution. |
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In situ hybridization
Use at an assay dependent dilution. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteinase K (ab64220)ab64220 Proteinase K used with aa anti-Pan CK antibody on Human skin
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab64220 has been referenced in 14 publications.
- Khoury EE et al. Pulmonary, cardiac and renal distribution of ACE2, furin, TMPRSS2 and ADAM17 in rats with heart failure: Potential implication for COVID-19 disease. J Cell Mol Med 25:3840-3855 (2021). PubMed: 33660945
- Kutlu T et al. Tyrosol retards induction of fibrosis in rats. J Food Biochem 45:e13965 (2021). PubMed: 34636053
- Wang K et al. Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA. Sci Adv 6:eaba7606 (2020). PubMed: 32832668
- Gleditsch K et al. Intratumoral Translocation Positive Heterogeneity in Pediatric Alveolar Rhabdomyosarcoma Tumors Correlates to Patient Survival Prognosis. Front Cell Dev Biol 8:564136 (2020). PubMed: 33072748
- Kole E et al. Effects of melatonin on ovarian reserve in cigarette smoking: an experimental study. Arch Med Sci 16:1376-1386 (2020). PubMed: 33224337