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Pluripotent stem cells can be used to generate hematopoietic stem and mature cells using in vitro differentiation protocols. Here we use human embryonic stem cells (hESCs) as the source of pluripotent stem cells and highlight the relevant cytokines and feeder cells necessary to differentiate them towards myeloid and lymphoid lineages.
BMP4, VEGF, FGF1, bFGF, SCF, Flt3-L, TPO, GM-CSF, IL-2, IL-4, IL-15, G-CSF, IL-3, IL-6, IL-7
IL-7, IL-3, SCF, Flt3-L
GM-CSF, IL-4, TNF-α
SCF, Flt3-L, GM-CSF, IL-3, TPO, IL-4, TNF-a
bFGF, VEGF, EPO, SCF, nFlt3-L, IL-3, IL-6, G-CSF, TPO
SCF, IL-3, IL-6, TPO, G-CSF, EPO
mFL stromal cells
EPO, TPO, IL-3, IL-6, Flt3-L, SCF, Dex
IGF-II, VEGF, SCF, Flt3-L, TPO, G-CSF
BMP4, VEGF, SCF, TPO, Flt3-L, IL-3, G-CSF
IL-3, G-CSF or GM-CSF
BMP4, VEGF, bFGF, SCF, TPO, IL-3
BMP4, VEGF, IL-3, Flt3-L, TPO, SCF, EPO
BMP4, VEGF, bFGF, TPO, SCF, Flt3-L, IL-3, IL-6, IL-9
SCF, Flt-3 ligand, IL-6, IL-6 receptor, thrombopoietin, IL-3, G-CSF
BMP4, bFGF, Activin A, VEGF, IGF-1, IL-6, IL-11, SCF, IL-3, EPO, TPO, IL-13, Flt3-L, IL-15
BMP4, VEGF, SCF, IL-3, Il-6, TPO, EPO, IL-7, Flt3-L, IL-15
Flt-3 ligand, IL-7, SCF
BMP4, bFGF, Activin A, VEGF, IL-6, IGF-1, IL-11, SCF, EPO, TPO, Flt3-L, IL-7, IL-15
BMP4, bFGF, Activin A, VEGF, IGF-1, IL-6, IL-11, SCF, IL-3, EPO, TPO, IL-3, Flt3-L, IL-7
BMP4, bFGF, VEGF, TPO, SCF, IL-6, IL-3, IL-7, Flt3-L
After differentiation, myeloid and lymphoid cells can be characterized by flow cytometry using specific cell surface markers from our immune cell markers poster.
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