For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

Array tomography protocol

Related

  • Protocol library
    • Limited edition protocols book

      Find out how to perform ultra-high resolution array tomography to uncover previously unseen details of brain molecular architecture.
      ​
      Full array tomography protocol

      Tissue fixation, dehydration and embedding​​​​​​

      Preparation of arrays​

      Immunostaining, elution and data analysis


      ​Array tomography is an ultra-high resolution imaging technique originally developed at Stanford University by Kristina D. Micheva and Stephen J. Smith in order to study neural circuits.

      Resin-embedded tissue is cut into ordered arrays of ultra-thin serial sections that are mounted onto microscope slides and stained with fluorescent antibodies. The arrays can be eluted and re-stained multiple times in order to reconstruct a three-dimensional image of antigen distribution.

      Array tomography offers multiplex, ultra-high resolution volumetric imaging with depth-independent immunofluorescent staining.

      ​​

      1. Tissue fixation, dehydration and embedding

      Discover how to fix, dehydrate and embed tissue for use with array tomography. Tissue fixation immobilizes antigens, whilst preserving molecular architecture. Fixed tissue is dehydrated through immersion in increasing concentrations of ethanol and the water replaced with an acrylic resin. The acrylic resin allows ultra-thin sectioning and repeated immunostaining.

      Follow our protocol


      2. Preparation of arrays

      Learn how to prepare arrays of serial sections from resin-embedded tissue. Coverslips are treated with a subbing solution to adhere serial sections. The embedded tissue is cut into ultra-thin sections, which are transferred onto coverslips and mounted onto microscopy slides for repeated immunostaining.​

      Follow our protocol


      3. Immunostaining, elution and data analysis

      Read about antibody staining of arrays, repeated elution and data analysis. Arrays are blocked and incubated with fluorescent antibodies before being imaged and analyzed. This process can be repeated multiple times to build a three dimensional image of antigen distribution within single tissue specimens.​

      Follow our protocol
      ​

      All protocols are courtesy of Kristina D. Micheva.

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.