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-Please note this protocol is no longer updated-
1. Culture adherent cells - motor neuron-enriched cultures from ventral spinal cord (13-day old mouse embryos)
2. Dissolve Fura2-AM
3. Add Fura-2 AM to your cells
5. Take out coverslip and transfer to imaging setup
6. Image cells
This protocol was kindly submitted by Dr Janin Lautenschläger. For more information please refer to Lautenschläger et al. Exper Neurol 2013, PMID: 23578819.