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Procedure for detection of caspases using immunofluorescence.
Apoptosis is a highly regulated mechanism of cell death, which converges on caspase activation. Immunofluorescence is a useful technique when you want to detect caspases and other apoptosis-related proteins simultaneously in a cell sample.
Caspase immunofluorescence protocol
*We recommend using serum from the host species of the secondary conjugate antibody (or closely related species) e.g. if using a goat anti-rabbit conjugate, use goat serum in the blocking buffer.
This protocol should be used as a guide. Optimization will be required depending on the sample and antibodies used. Our antibody datasheets provide suggested working concentrations which should be tested in your own experiments. Our range of caspase antibodies can be found here.
We also have a range of caspase staining kits for a simple and way of measuring active caspase in live cells. An optimized protocol is provided with each kit, and should be followed carefully.