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Please refer to the protocol included with your kit for product-specific details regarding sample preparation and compatible sample types.
This guide is intended to be an educational resource. These are general guidelines for preparing commonly tested samples for use in ELISA assays. As with all aspects of assay development, optimum sample preparation procedures will vary depending on the target and assay of interest. It is always good practice to consult the literature for experimental examples similar to your own when developing a new assay.
Cell culture supernatant
Volumes of lysis buffer must be determined in relation to the amount of tissue present. Typical concentration of final protein extract is >1 mg/mL.
Additional reagents required to produce complete extraction buffer.
Supplement the cell extraction buffer with phosphatase and protease inhibitor cocktails as described by manufacturer, and PMSF to 1 mM, immediately before use.
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