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Discover the two procedures, soluble (DEA) and insoluble (FA), for the extraction of amyloid beta from mouse brain.
This protocol is for the extraction of two fractions; soluble (extracted using diethylamine, DEA) and insoluble (extracted with formic acid, FA).
DEA extraction isolates non-plaque-associated amyloid beta.
FA extraction isolates deposited plaque-associated amyloid beta.
FA neutralization solution: (1 M Tris base, 0.5 M Na2HPO4, 0.05% NaN3)