Immunohistochemistry (IHC) application guide
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Easily plan and optimize your IHC experiments with our comprehensive guide.
The booklet includes tips and guidelines on:
- Sample preparation (fixation and embedding)
- Tissue treatment and antigen retrieval
- Blocking methods (eg for endogenous peroxidase and alkaline phosphatase)
- Selecting the right immunostaining/detection system and reagents
- Which chromogen/substrate to use
- Recommended cancer and neuroscience markers
- And much more ...
Alternatively, you can read the online version of our guide to IHC.
Get results fast with validated kits and reagents
For IHC, to ensure that you get the data you need on your first experiment, we recommend our optimized kits and reagents:
- Buffers for heat-mediated and enzymatic antigen retrieval
- Highly sensitive Labeled Streptavidin-Biotin LSAB ABC kits and micro-polymer EXPOSE kits for IHC detection and amplification (for mouse antibodies used on mouse samples, we recommend our Mouse-on-Mouse kit)
- Biotin and micro-polymer conjugated secondary antibodies to construct your own ABC method
- Optimized normal serums for blocking
- IHC validated primary antibodies