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Convenient kits for nuclear extraction and fractionation
For reliable results when extracting the nuclear fraction, we recommend our nuclear extraction kit (ab219177).
Alternatively, we use the protocol below.
All centrifugation should be done at 4°C. Samples should be kept on ice throughout the procedure.
Nuclear extraction and fractionation buffer
|MW||mM||Add per L||Unit|
|HEPES (pH 7.4)||238.30||20||4.77||g|
Just before use, add the following per 10 mL:
|Stocks||Per 50 mL|
|1 mM DTT||1M||10 µL|
|PI Cocktail (III)||-||50 µL|