For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Take a look at our BETA site and see what we’ve done so far.
Search and browse selected products
Purchase these through your usual distributor
Convenient kits for nuclear extraction and fractionation
For reliable results when extracting the nuclear fraction, we recommend our nuclear extraction kit (ab219177).
Alternatively, we use the protocol below.
All centrifugation should be done at 4°C. Samples should be kept on ice throughout the procedure.
|Mw||mM||Add per L|
|HEPES (pH 7.4)||238.30||20||4.77 g|
Just before use, add the following per 10 mL:
|Stocks||Per 10 mL|
|1 mM DTT||1M||10 µL|
|PI Cocktail (III)||-||50 µL|