For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Take a look at our BETA site and see what we’ve done so far.
Search and browse selected products
Purchase these through your usual distributor
In many cell lines, MMP-9 is expressed at low levels. However, treatment with phorbol myristate acetate (PMA) – a protein kinase C ligand – markedly stimulates MMP9 expression (Roomi et al., 2009). For analysis of MMP-9 in cell lines, it may be necessary to first stimulate your cells with PMA to detect a signal.
The following protocol has been optimized for use with U937 cells.
Lysate can either be stored at -80°C or used immediately for western blotting.
Add proteinase inhibitor before use.
NaCl | 150 mM |
NP-40 or Triton X-100 | 1.0% |
Sodium deoxychlorate | 0.5% |
SDS (sodium dodecyl sulfate) | 0.1% |
Tris, pH 8.0 | 50 mM |