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|NB Avoid touching the membrane with the pipette tip. Let pipetted RNA droplet diffuse onto the membrane via surface tension. Change tips after each loading, even between the same sample.|
|NB If oligos are biotinylated, probe with a Streptavidin-AlexaFluor as a loading control. Ensure the ECL from the previous imaging has been quenched; thorough washing will aid this.|
|NB For loading control of non-biotinylated RNA either methylene blue or ethidium bromide can be used|