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Mitochondrial lysis buffer
25 mM HEPES KOH, pH 7.6
5 mM MgCl2
0.5 mM EDTA
1 mM DTT
1 mM PMSF
This is a modified protocol taken from: Micol V, Fernandez-Silva P and Attardi G (1996). Mitochondrial Biogenesis and Genetics Part B. Methods of Enzymology, Volume 264, p 3–621.
For convenience please consider our Mitochondria Isolation Kit for Cultured Cells (ab110171).