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|1 mM CaCl2||26 µl|
|1 mM MgCl2||22 µl|
10% pluronic F-127 (DMSO)
4 mg/ml Fluo-3 AM (DMSO)
10 mg/ml Fura Red AM (DMSO)
This protocol is adapted from Huang, G. N. (2012). T cell Calcium Mobilization Study (Flow Cytometry). Bio-protocol 2(9): e171. http://www.bio-protocol.org/e171