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Video protocols

Related

Ensure standardization and successful replication of results with our easy-to-follow video protocols.

1.   Brain slice electrophysiology
2.   IHC for brain slice sections
3.   IHC for paraffin-embedded sections
4.   Cryopreservation of mammalian cells lines
5.   Aseptic technique
6.   Sectioning of paraffin-embedded tissue
7.   Western blot
8.   ICC/IF
9.   Counting cells using a hemocytometer

​ 

Brain slice sections video protocol

This protocol describes the preparation of hippocampal slice and electrophysiology recording.

Watch our 12-minute video protocol here.


IHC for brain slice sections video protocol

Learn how to visualize your proteins of interest with IHC on brain slice sections after electrophysiology.

Watch our 4-minute video protocol here.


IHC for paraffin-embedded sections video protocol 

This protocol demonstrates immunohistochemistry (IHC) with paraffin sections, using a variety of antibodies and stains to reveal the molecular architecture of the tissue.

Watch our 5-minute video protocol here.


Cryopreservation of mammalian cell lines video protocol

Cryopreservation is a method whereby cells are frozen, maintaining viability, until they are defrosted months or years later. Cells are cryopreserved to minimize genetic change and avoid loss through contamination.

Watch our 3-minute video protocol here.


Aseptic technique video protocol

This protocol demonstrates how to sterilize work areas and use appropriate sterile handling techniques, personal protective equipment, and good hygiene.

Watch our 2-minute video protocol here.


Sectioning of paraffin-embedded tissue video protocol 

This protocol describes how to cut sections from tissue embedded in paraffin blocks.

Watch our 3-minute video protocol here.


Western blot video protocol

Our western blot protocol includes solutions and reagents, procedure, and useful links to guide you through your experiment.

Watch our 8-minute video protocol here.

western blot protocol

​​

ICC/IF video protocol

Find out how to use fluorescent antibodies or dyes to detect target antigens within cells.

Watch our 7-minute video protocol here.


Counting cells using a hemocytometer video protocol 

This protocol demonstrates how to obtain a viable cell count from suspension cells using a hemocytometer.

Watch our 7-minute video protocol here.


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