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1. Brain slice electrophysiology
2. IHC for brain slice sections
3. IHC for paraffin-embedded sections
4. Cryopreservation of mammalian cells lines
5. Aseptic technique
6. Sectioning of paraffin-embedded tissue
7. Western blot
8. ICC/IF
9. Counting cells using a hemocytometer
This protocol describes the preparation of hippocampal slice and electrophysiology recording.
Watch our 12-minute video protocol here.
Learn how to visualize your proteins of interest with IHC on brain slice sections after electrophysiology.
Watch our 4-minute video protocol here.
This protocol demonstrates immunohistochemistry (IHC) with paraffin sections, using a variety of antibodies and stains to reveal the molecular architecture of the tissue.
Watch our 5-minute video protocol here.
Cryopreservation is a method whereby cells are frozen, maintaining viability, until they are defrosted months or years later. Cells are cryopreserved to minimize genetic change and avoid loss through contamination.
Watch our 3-minute video protocol here.
This protocol demonstrates how to sterilize work areas and use appropriate sterile handling techniques, personal protective equipment, and good hygiene.
Watch our 2-minute video protocol here.
This protocol describes how to cut sections from tissue embedded in paraffin blocks.
Watch our 3-minute video protocol here.
Our western blot protocol includes solutions and reagents, procedure, and useful links to guide you through your experiment.
Watch our 8-minute video protocol here.
Find out how to use fluorescent antibodies or dyes to detect target antigens within cells.
Watch our 7-minute video protocol here.
This protocol demonstrates how to obtain a viable cell count from suspension cells using a hemocytometer.
Watch our 7-minute video protocol here.