Anti-PROX1 antibody (ab38692)
Key features and details
- Rabbit polyclonal to PROX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PROX1 antibody
See all PROX1 primary antibodies -
Description
Rabbit polyclonal to PROX1 -
Host species
Rabbit -
Specificity
We have had mixed results for use of this antibody in IHC-P. The AbReview below from an external researcher reports good staining with this antibody with human heart tissue, but we have had mixed results with this antibody in internal QC testing in IHC-P. We welcome any feedback from customers who have used this antibody in IHC-P.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human PROX1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab38691)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| All applications |
Mouse
Rat
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).
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| Notes |
|---|
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa). |
Target
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Function
Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3. -
Tissue specificity
Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the Prospero homeobox family.
Contains 1 Prospero-type homeobox DNA-binding domain. -
Domain
The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity. -
Cellular localization
Nucleus. RORG promotes its nuclear localization. - Information by UniProt
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Database links
- Entrez Gene: 5629 Human
- Entrez Gene: 19130 Mouse
- Entrez Gene: 305066 Rat
- Omim: 601546 Human
- SwissProt: Q92786 Human
- SwissProt: P48437 Mouse
- Unigene: 741808 Human
- Unigene: 744931 Human
see all -
Form
Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter. -
Alternative names
- Homeobox prospero like protein antibody
- Homeobox prospero like protein PROX1 antibody
- Homeobox prospero-like protein PROX1 antibody
see all
Images
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Western blot - Anti-PROX1 antibody (ab38692)All lanes : Anti-PROX1 antibody (ab38692) at 1 µg/ml (3% Milk Block)
Lane 1 : Hep G2 (Human) Whole Cell Lysate (ab52257)
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate (ab45968)
Lane 3 : HEK293 (Human) Whole Cell Lysate (ab52256)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
References (8)
ab38692 has been referenced in 8 publications.
- Parmhans N et al. Characterization of retinal ganglion cell, horizontal cell, and amacrine cell types expressing the neurotrophic receptor tyrosine kinase Ret. J Comp Neurol 526:742-766 (2018). PubMed: 29218725
- Zhang Y et al. Crim1 regulates integrin signaling in murine lens development. Development 143:356-66 (2016). IHC . PubMed: 26681494
- Mori M et al. Pulmonary and pleural lymphatic endothelial cells from pediatric, but not adult, patients with Gorham-Stout disease and generalized lymphatic anomaly, show a high proliferation rate. Orphanet J Rare Dis 11:67 (2016). IHC . PubMed: 27194137
- Osborn AJ et al. Activating PIK3CA alleles and lymphangiogenic phenotype of lymphatic endothelial cells isolated from lymphatic malformations. Hum Mol Genet 24:926-38 (2015). PubMed: 25292196
- Davis JM et al. Lymphatic endothelial differentiation in pulmonary lymphangioleiomyomatosis cells. J Histochem Cytochem 61:580-90 (2013). Human . PubMed: 23609227
- Lecompte S et al. Genetic and molecular insights into the role of PROX1 in glucose metabolism. Diabetes 62:1738-45 (2013). PubMed: 23274905
- Shimoda H & Isogai S Immunohistochemical demonstration of lymphatic vessels in adult zebrafish. Acta Histochem Cytochem 45:335-41 (2012). IHC . PubMed: 23378677
- Dellinger MT & Brekken RA Phosphorylation of Akt and ERK1/2 Is Required for VEGF-A/VEGFR2-Induced Proliferation and Migration of Lymphatic Endothelium. PLoS One 6:e28947 (2011). ICC/IF ; Human . PubMed: 22174934
