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    prox1-antibody-ab38692.pdf

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Anti-PROX1 antibody (ab38692)

  • Datasheet
  • SDS
Reviews (8)Q&A (4)References (8)

Product price, shipping and contact information

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Abpromise

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Find out more.

Western blot - Anti-PROX1 antibody (ab38692)

    Key features and details

    • Rabbit polyclonal to PROX1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-PROX1 antibody
      See all PROX1 primary antibodies
    • Description

      Rabbit polyclonal to PROX1
    • Host species

      Rabbit
    • Specificity

      We have had mixed results for use of this antibody in IHC-P. The AbReview below from an external researcher reports good staining with this antibody with human heart tissue, but we have had mixed results with this antibody in internal QC testing in IHC-P. We welcome any feedback from customers who have used this antibody in IHC-P.

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human PROX1 aa 150-250 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab38691)

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Neural Signal Transduction
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Developmental Families
      • Other
      • Cardiovascular
      • Heart
      • Cardiogenesis
      • Transcription factors/regulators

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human PROX1 protein (ab54337)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab38692 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Application Abreviews Notes
    WB (2)
    Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).
    Notes
    WB
    Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).

    Target

    • Function

      Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
    • Tissue specificity

      Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
    • Sequence similarities

      Belongs to the Prospero homeobox family.
      Contains 1 Prospero-type homeobox DNA-binding domain.
    • Domain

      The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.
    • Cellular localization

      Nucleus. RORG promotes its nuclear localization.
    • Target information above from: UniProt accession Q92786 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5629 Human
      • Entrez Gene: 19130 Mouse
      • Entrez Gene: 305066 Rat
      • Omim: 601546 Human
      • SwissProt: Q92786 Human
      • SwissProt: P48437 Mouse
      • Unigene: 741808 Human
      • Unigene: 744931 Human
      • Unigene: 132579 Mouse
      • Unigene: 392678 Mouse
      • Unigene: 26557 Rat
      see all
    • Form

      Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter.
    • Alternative names

      • Homeobox prospero like protein antibody
      • Homeobox prospero like protein PROX1 antibody
      • Homeobox prospero-like protein PROX1 antibody
      • Prospero homeobox 1 antibody
      • Prospero homeobox protein 1 antibody
      • Prospero related homeobox 1 antibody
      • prospero-related homeobox gene 1 antibody
      • PROX 1 antibody
      • PROX-1 antibody
      • PROX1 antibody
      • PROX1_HUMAN antibody
      • zgc:111888 antibody
      see all

    Images

    • Western blot - Anti-PROX1 antibody (ab38692)
      Western blot - Anti-PROX1 antibody (ab38692)
      All lanes : Anti-PROX1 antibody (ab38692) at 1 µg/ml (3% Milk Block)

      Lane 1 : Hep G2 (Human) Whole Cell Lysate (ab52257)
      Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate (ab45968)
      Lane 3 : HEK293 (Human) Whole Cell Lysate (ab52256)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 83 kDa
      Observed band size: 95 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (8)

    Publishing research using ab38692? Please let us know so that we can cite the reference in this datasheet.

    ab38692 has been referenced in 8 publications.

    • Parmhans N  et al. Characterization of retinal ganglion cell, horizontal cell, and amacrine cell types expressing the neurotrophic receptor tyrosine kinase Ret. J Comp Neurol 526:742-766 (2018). PubMed: 29218725
    • Zhang Y  et al. Crim1 regulates integrin signaling in murine lens development. Development 143:356-66 (2016). IHC . PubMed: 26681494
    • Mori M  et al. Pulmonary and pleural lymphatic endothelial cells from pediatric, but not adult, patients with Gorham-Stout disease and generalized lymphatic anomaly, show a high proliferation rate. Orphanet J Rare Dis 11:67 (2016). IHC . PubMed: 27194137
    • Osborn AJ  et al. Activating PIK3CA alleles and lymphangiogenic phenotype of lymphatic endothelial cells isolated from lymphatic malformations. Hum Mol Genet 24:926-38 (2015). PubMed: 25292196
    • Davis JM  et al. Lymphatic endothelial differentiation in pulmonary lymphangioleiomyomatosis cells. J Histochem Cytochem 61:580-90 (2013). Human . PubMed: 23609227
    • Lecompte S  et al. Genetic and molecular insights into the role of PROX1 in glucose metabolism. Diabetes 62:1738-45 (2013). PubMed: 23274905
    • Shimoda H & Isogai S Immunohistochemical demonstration of lymphatic vessels in adult zebrafish. Acta Histochem Cytochem 45:335-41 (2012). IHC . PubMed: 23378677
    • Dellinger MT & Brekken RA Phosphorylation of Akt and ERK1/2 Is Required for VEGF-A/VEGFR2-Induced Proliferation and Migration of Lymphatic Endothelium. PLoS One 6:e28947 (2011). ICC/IF ; Human . PubMed: 22174934

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PROX1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Permeabilization
    No
    Specification
    testis
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 18 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PROX1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (gut)
    Antigen retrieval step
    Heat mediated
    Specification
    gut
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 29 2015

    Question

    - testing discount for ab38692 in pig (IHC-P)

    Read More

    Abcam community

    Verified customer

    Asked on Aug 29 2012

    Answer



    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab38692 in Schweinegewebezu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer xx, deckt den Wert von ab38692 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

    Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test inSchweinegewebe eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

    Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

    Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

    Read More

    Abcam Scientific Support

    Answered on Aug 29 2012

    Question

    IHC-P on pig
    for Podoplanin, CD31, PRox1, vWF and CD34
    Podoplanin:ab38692 100% homology
    CD31: ab76533 or ab134168 no alingment

    Read More

    Abcam community

    Verified customer

    Asked on Aug 17 2012

    Answer

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Leider sind Schwein und Maus oder Humanes Proteinfür Anti-Podoplanin / gp36 und CD31 nicht sehr homolog. Deshalb kann ich Ihnen auch keine Antikörper empfehlen.

    ab68545 (Anti-Von Willebrand Factor antibody [2Q2134]) und ab81289 (Anti-CD34 antibody [EP373Y])sind für Schwein getestet und garantiert.

    Unserem Wissen nach wurde ab38692 bisher noch nicht inSchwein getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab38692 abdecken, und gegen eine erneute Bestellung von uns verrechnet werden.

    Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

    1.) Bestätigen Sie mir bitte, dass Sie ab38692 in Schwein testen möchten.

    2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

    3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

    4.) Testen Sie den Antikörper in Schwein.

    5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
    Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
    https://www.abcam.com/abreviews

    6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können ein anderes Produkt zu dem gleichen Preis wie ab38692 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab38692 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

    Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

    Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
    https://www.abcam.com/collaborationdiscount

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Read More

    Abcam Scientific Support

    Answered on Aug 17 2012

    Western blot abreview for Anti-PROX1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (mouse heart)
    Loading amount
    50 µg
    Specification
    mouse heart
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 27 2012

    Question

    Inquiry: Want to order Prox1 antibody but need prices and how it comes. (1mL concentrate?). Please e-mail the info. Thank you

    Read More

    Abcam community

    Verified customer

    Asked on Jun 04 2012

    Answer

    Thank you for contacting us.

    The anti-PROX1 antibody ab38692 is $347 for 100 µg+ $41 shipping and handling in the US. The antibody comes as a liquid, 100 µg at 1mg/ml, so you receive 100 µl.

    https://www.abcam.com/prox1-antibody-ab38692.html


    If you would like to order it in bulk and need a quote, please email mailto:sales@abcam.com.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jun 04 2012

    Question

    Bonjour, L’anticorps anti-PROX I ab38692 commandé en Août, ne sera pas disponible avant décembre. Auriez-vous un produit alternatif à nous proposer ? Cordialement.  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 20 2011

    Answer

    Merci pour votre émail. Nous avons plusieurs alternatives pour cet anticorps, mais ça dépends dans quel application et sur quel espèce le client veux détecter le PROX I. Le ab33219 est probablement le meilleur choix de remplacement, pourvu que le client veux bien un anticorps monoclonal de souris à la place d'un anticorps polylconal de lapin. Le ab33219 est testé  en IHC-P et WB (comme le ab38692) et sur du tissue de rat et humain (pas souris encore).  Click here (or use the following: https://www.abcam.com/PROX1-antibody-5G10-ab33219.html). Si nous avons pas un anticorps testé dans l'espèce et l'application qu'il souhaite, nous pouvons vous faire probablement notre offre de test (www.abcam.com/collaborationdiscount) Le ab37128, est testé sur les mêmes espèces (rat, souris, humain), mais pas en Western blot, ni en coupes paraffines. Le ab57746, ab101851 et ab104629 sont tous testées en Western blot et sur du tissue humain. Le ab104629 aussi sur le tissue de souris. J'espère que cette information est utile pour vous et que nous pouvons trouver un remplacement pour le client. N'hésitez pas à me recontacter si vous avez besoin de plus de renseignement.  

    Read More

    Abcam Scientific Support

    Answered on Oct 20 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-PROX1 antibody

    Poor
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E9.5 EMBRYONIC PROEPICARDIUM AND ADJACENT LIVER BU)
    Specification
    E9.5 EMBRYONIC PROEPICARDIUM AND ADJACENT LIVER BU
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 1% Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Read More

    Dr. Stephanie Cossette

    Verified customer

    Submitted Oct 05 2011

    Western blot abreview for Anti-PROX1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (P3 Dentate Gyrus)
    Loading amount
    50 µg
    Specification
    P3 Dentate Gyrus
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Ms. Xia Chen

    Verified customer

    Submitted Apr 01 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PROX1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Heart)
    Specification
    Heart
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More

    Mr. Antibody Solutions

    Verified customer

    Submitted Apr 08 2010

    1-10 of 12 Abreviews or Q&A

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