Anti-PROX1 antibody (ab38692)
- Datasheet
- References (7)
- Protocols
Overview
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Product nameAnti-PROX1 antibody
See all PROX1 primary antibodies -
DescriptionRabbit polyclonal to PROX1
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Host speciesRabbit
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SpecificityWe have had mixed results for use of this antibody in IHC-P. The abReview below from an external researcher reports good staining with this antibody with human heart tissue, but we have had mixed results with this antibody in internal QC testing in IHC-P. Thus, we are removing IHC-P as a guaranteed application and welcome any feedback from customers who have used this antibody in IHC-P.
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human PROX1.
Read Abcam's proprietary immunogen policy (Peptide available as ab38691.) -
Positive control
- This antibody gave a positive signal in the following human whole cell lysates : HepG2 and SHSY-5Y. HEK293 human whole cell lysate is a negative control lysate. IHC-P: Human heart tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab38692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa). | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionTranscription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
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Tissue specificityMost actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
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Sequence similaritiesBelongs to the Prospero homeobox family.
Contains 1 Prospero-type homeobox DNA-binding domain. -
DomainThe prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.
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Cellular localizationNucleus. RORG promotes its nuclear localization.
- Information by UniProt
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Database links
- Entrez Gene: 5629 Human
- Entrez Gene: 19130 Mouse
- Entrez Gene: 305066 Rat
- Omim: 601546 Human
- SwissProt: Q92786 Human
- SwissProt: P48437 Mouse
- Unigene: 741808 Human
- Unigene: 744931 Human
see all -
FormProx1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter.
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Alternative names
- Homeobox prospero like protein antibody
- Homeobox prospero like protein PROX1 antibody
- Homeobox prospero-like protein PROX1 antibody
see all
Images
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Western blot - Anti-PROX1 antibody (ab38692)All lanes : Anti-PROX1 antibody (ab38692) at 1 µg/ml (3% Milk Block)
Lane 1 : HepG2 (Human) Whole Cell Lysate (ab52257)
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate (ab45968)
Lane 3 : HEK293 (Human) Whole Cell Lysate (ab52256)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PROX1 antibody (ab38692)Formaldehyde-fixed, paraffin-embedded human heart tissue stained for PROX1 using ab38692 at 1/250 dilution in immunohistochemical analysis. Incubation with primary antibody was performed for 45 minutes at 20°C. Goat anti-rabbit/mouse HRO polymer was used as a secondary antibody.
Heat mediated antigen retrieval was performed. Tissue sections were blocked with peroxidase for 5 minutes followed by a 10 minute protein block.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhang Y et al. Crim1 regulates integrin signaling in murine lens development. Development 143:356-66 (2016). IHC . Read more (PubMed: 26681494) »
- Mori M et al. Pulmonary and pleural lymphatic endothelial cells from pediatric, but not adult, patients with Gorham-Stout disease and generalized lymphatic anomaly, show a high proliferation rate. Orphanet J Rare Dis 11:67 (2016). IHC . Read more (PubMed: 27194137) »
