Overview

  • Product name
  • Description
    Rabbit polyclonal to PROX1
  • Host species
    Rabbit
  • Specificity
    We have had mixed results for use of this antibody in IHC-P. The abReview below from an external researcher reports good staining with this antibody with human heart tissue, but we have had mixed results with this antibody in internal QC testing in IHC-P. Thus, we are removing IHC-P as a guaranteed application and welcome any feedback from customers who have used this antibody in IHC-P.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human PROX1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab38691.)

  • Positive control
    • This antibody gave a positive signal in the following human whole cell lysates : HepG2 and SHSY-5Y. HEK293 human whole cell lysate is a negative control lysate. IHC-P: Human heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab38692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
  • Tissue specificity
    Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the Prospero homeobox family.
    Contains 1 Prospero-type homeobox DNA-binding domain.
  • Domain
    The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.
  • Cellular localization
    Nucleus. RORG promotes its nuclear localization.
  • Information by UniProt
  • Database links
  • Form
    Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter.
  • Alternative names
    • Homeobox prospero like protein antibody
    • Homeobox prospero like protein PROX1 antibody
    • Homeobox prospero-like protein PROX1 antibody
    • Prospero homeobox 1 antibody
    • Prospero homeobox protein 1 antibody
    • Prospero related homeobox 1 antibody
    • prospero-related homeobox gene 1 antibody
    • PROX 1 antibody
    • PROX-1 antibody
    • PROX1 antibody
    • PROX1_HUMAN antibody
    • zgc:111888 antibody
    see all

Images

  • All lanes : Anti-PROX1 antibody (ab38692) at 1 µg/ml (3% Milk Block)

    Lane 1 : HepG2 (Human) Whole Cell Lysate (ab52257)
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate (ab45968)
    Lane 3 : HEK293 (Human) Whole Cell Lysate (ab52256)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 83 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • Formaldehyde-fixed, paraffin-embedded human heart tissue stained for PROX1 using ab38692 at 1/250 dilution in immunohistochemical analysis. Incubation with primary antibody was performed for 45 minutes at 20°C. Goat anti-rabbit/mouse HRO polymer was used as a secondary antibody.

    Heat mediated antigen retrieval was performed. Tissue sections were blocked with peroxidase for 5 minutes followed by a 10 minute protein block.

    See Abreview

References

This product has been referenced in:
  • Zhang Y  et al. Crim1 regulates integrin signaling in murine lens development. Development 143:356-66 (2016). IHC . Read more (PubMed: 26681494) »
  • Mori M  et al. Pulmonary and pleural lymphatic endothelial cells from pediatric, but not adult, patients with Gorham-Stout disease and generalized lymphatic anomaly, show a high proliferation rate. Orphanet J Rare Dis 11:67 (2016). IHC . Read more (PubMed: 27194137) »
See all 7 Publications for this product

Customer reviews and Q&As

11-12 of 12 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No

Dr. Sophie Pezet

Verified customer

Submitted Mar 03 2010

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No

Dr. Sophie Pezet

Verified customer

Submitted Feb 23 2010

11-12 of 12 Abreviews or Q&A

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