Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PROX1 antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Other product details

Dilution
1/250
Incubation time
45 minute(s) · Temperature: 20°C · Diluent: Antibody Diluent (Dako)

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit/mouse HRP polymer (pre-diluted, Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
Some very specific nuclear staining with minimal background.

Mr. Antibody Solutions

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Submitted Apr 08 2010

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