Immunohistochemistry (Frozen sections) abreview for Anti-PROX1 antibody

Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E9.5 EMBRYONIC PROEPICARDIUM AND ADJACENT LIVER BU)
Specification
E9.5 EMBRYONIC PROEPICARDIUM AND ADJACENT LIVER BU
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexa 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/250

Additional data

Additional Notes
I purchased this antibody as a replacement for the discontinued ab11941
Unfortunately I could not get the antibody to work. I repeated the experiment using multiple concentration and on different freshly harvested and sectioned tissue and there was still no positive staining.
If you are looking for a replacement for ab11941 I would strongly recommend ab37128
Abcam response
IHC-Fr is an un-tested application for ab38692.
This user took part in our testing discount program and received a free antibody for their valuable feedback.
We hope that this information will be helpful for future users and would like to receive more feedback from users of this antibody.

Dr. Stephanie Cossette

Verified customer

Submitted Oct 05 2011

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