Western blot abreview for Anti-PROX1 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
50 µg
Specification
mouse heart
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: 5%milk

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ecl
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: two bands, 55, 60 kDa

Additional data

Additional Notes
50 ug of homogenized mouse heart tissue were loaed onto 10% SDS-PAGE. Anti-prox1 antibody was diluted at 1:1000
anti-rabbit IgG-HRP secondary antibody was diluted at 1:4000
two bands (55kd and 60 kd) were observed, but no predicted 110kd band was found in this mouse heart sample.

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Submitted Jul 27 2012

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