50 ug of homogenized mouse heart tissue were loaed onto 10% SDS-PAGE. Anti-prox1 antibody was diluted at 1:1000 anti-rabbit IgG-HRP secondary antibody was diluted at 1:4000 two bands (55kd and 60 kd) were observed, but no predicted 110kd band was found in this mouse heart sample.
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Submitted Jul 27 2012
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