Recombinant
RabMAb

Recombinant Anti-PROX1 antibody [EPR3128(2)] - BSA and Azide free (ab196728)

Overview

  • Product name

    Anti-PROX1 antibody [EPR3128(2)] - BSA and Azide free
    See all PROX1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3128(2)] to PROX1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PROX1. The exact sequence is proprietary.

  • Positive control

    • HepG2 and SHSY-5Y cell lysates
  • General notes

    ab196728 is the carrier-free version of ab124910 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab196728 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 83 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
    • Tissue specificity

      Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
    • Sequence similarities

      Belongs to the Prospero homeobox family.
      Contains 1 Prospero-type homeobox DNA-binding domain.
    • Domain

      The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.
    • Cellular localization

      Nucleus. RORG promotes its nuclear localization.
    • Information by UniProt
    • Database links

    • Form

      Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter.
    • Alternative names

      • Homeobox prospero like protein antibody
      • Homeobox prospero like protein PROX1 antibody
      • Homeobox prospero-like protein PROX1 antibody
      • Prospero homeobox 1 antibody
      • Prospero homeobox protein 1 antibody
      • Prospero related homeobox 1 antibody
      • prospero-related homeobox gene 1 antibody
      • PROX 1 antibody
      • PROX-1 antibody
      • PROX1 antibody
      • PROX1_HUMAN antibody
      • zgc:111888 antibody
      see all

    References

    ab196728 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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