Anti-PRP16 antibody (ab70781)
Key features and details
- Rabbit polyclonal to PRP16
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PRP16 antibody
See all PRP16 primary antibodies -
Description
Rabbit polyclonal to PRP16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to Human PRP16 aa 1200-1227 (C terminal).
Database link: NP_054722.2 -
Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70781 was affinity purified using an epitope specific to PRP16 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab70781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 140 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Probable ATP-binding RNA helicase involved in pre-mRNA splicing. -
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 520046 Cow
- Entrez Gene: 9785 Human
- Entrez Gene: 64340 Mouse
- Entrez Gene: 292007 Rat
- GenBank: NM_014003 Human
- Omim: 605584 Human
- SwissProt: Q17R09 Cow
- SwissProt: Q92620 Human
see all -
Alternative names
- ATP dependent RNA helicase DHX38 antibody
- ATP-dependent RNA helicase DHX38 antibody
- DDX38 antibody
see all
Images
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Western blot - Anti-PRP16 antibody (ab70781)All lanes : Anti-PRP16 antibody (ab70781) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 140 kDa
Observed band size: 140 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP16 antibody (ab70781)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRP16 with ab70781 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-PRP16 antibody (ab70781)Detection of PRP16 by Western Blot of Immunprecipitate. ab70781 at 1µg/ml staining PRP16 in HeLa whole cell lysate immunoprecipitated using ab70781 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
References (1)
ab70781 has been referenced in 1 publication.
- Shambharkar PB et al. TMEM203 Is a Novel Regulator of Intracellular Calcium Homeostasis and Is Required for Spermatogenesis. PLoS One 10:e0127480 (2015). PubMed: 25996873
