Anti-PRP16 antibody (ab70781)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-PRP16 antibody
See all PRP16 primary antibodies -
Description
Rabbit polyclonal to PRP16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to Human PRP16 aa 1200-1227 (C terminal).
Database link: NP_054722.2 -
Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70781 was affinity purified using an epitope specific to PRP16 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab70781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 140 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Probable ATP-binding RNA helicase involved in pre-mRNA splicing. -
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 520046 Cow
- Entrez Gene: 9785 Human
- Entrez Gene: 64340 Mouse
- Entrez Gene: 292007 Rat
- GenBank: NM_014003 Human
- Omim: 605584 Human
- SwissProt: Q17R09 Cow
- SwissProt: Q92620 Human
see all -
Alternative names
- ATP dependent RNA helicase DHX38 antibody
- ATP-dependent RNA helicase DHX38 antibody
- DDX38 antibody
see all
Images
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Western blot - Anti-PRP16 antibody (ab70781)All lanes : Anti-PRP16 antibody (ab70781) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 140 kDa
Observed band size: 140 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP16 antibody (ab70781)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRP16 with ab70781 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-PRP16 antibody (ab70781)Detection of PRP16 by Western Blot of Immunprecipitate. ab70781 at 1µg/ml staining PRP16 in HeLa whole cell lysate immunoprecipitated using ab70781 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
Datasheets and documents
References
This product has been referenced in:
- Shambharkar PB et al. TMEM203 Is a Novel Regulator of Intracellular Calcium Homeostasis and Is Required for Spermatogenesis. PLoS One 10:e0127480 (2015). Read more (PubMed: 25996873) »
