Overview

  • Product name

  • Description

    Rabbit polyclonal to PRP16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human PRP16 aa 1200-1227 (C terminal).
    Database link: NP_054722.2

  • Positive control

    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab70781 was affinity purified using an epitope specific to PRP16 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 140 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Probable ATP-binding RNA helicase involved in pre-mRNA splicing.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent RNA helicase DHX38 antibody
    • ATP-dependent RNA helicase DHX38 antibody
    • DDX38 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38 antibody
    • DEAH (Asp-Glu-Ala-His) box polypeptide 38 antibody
    • DEAH box protein 38 antibody
    • DHX38 antibody
    • KIAA0224 antibody
    • Pre mRNA splicing factor ATP dependent RNA helicase PRP16 antibody
    • Pre-mRNA splicing factor 16 antibody
    • Pre-mRNA-splicing factor ATP-dependent RNA helicase prp16 antibody
    • Prp16 antibody
    • PRP16 homolog of S.cerevisiae; pre-mRNA splicing factor ATP-dependent RNA helicase PRP16 antibody
    • PRP16_HUMAN antibody
    • PRPF16 antibody
    see all

Images

  • All lanes : Anti-PRP16 antibody (ab70781) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 140 kDa
    Observed band size: 140 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRP16 with ab70781 at 1/200 (1µg/ml). Detection: DAB.

  • Detection of PRP16 by Western Blot of Immunprecipitate. ab70781 at 1µg/ml staining PRP16 in HeLa whole cell lysate immunoprecipitated using ab70781 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

References

This product has been referenced in:

  • Shambharkar PB  et al. TMEM203 Is a Novel Regulator of Intracellular Calcium Homeostasis and Is Required for Spermatogenesis. PLoS One 10:e0127480 (2015). Read more (PubMed: 25996873) »
See 1 Publication for this product

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