Recombinant Anti-PRP16 antibody [EPR9845] (ab154801)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9845] to PRP16
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PRP16 antibody [EPR9845]
See all PRP16 primary antibodies -
Description
Rabbit monoclonal [EPR9845] to PRP16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PRP16. The exact sequence is proprietary.
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Positive control
- Raji, Jurkat, K562, 293T and HeLa cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9845 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 141 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 141 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Probable ATP-binding RNA helicase involved in pre-mRNA splicing. -
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9785 Human
- GenBank: NM_014003 Human
- Omim: 605584 Human
- SwissProt: Q92620 Human
- Unigene: 151218 Human
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Alternative names
- ATP dependent RNA helicase DHX38 antibody
- ATP-dependent RNA helicase DHX38 antibody
- DDX38 antibody
see all
Images
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All lanes : Anti-PRP16 antibody [EPR9845] (ab154801) at 1/1000 dilution
Lane 1 : Raji lysate
Lane 2 : Jurkat lysate
Lane 3 : K562 lysate
Lane 4 : 293T lysate
Lane 5 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 141 kDa -
Immunofluorescence analysis of HeLa cells labeling PRP16 with ab154801 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154801 has been referenced in 1 publication.
- Shakyawar DK et al. C3G dynamically associates with nuclear speckles and regulates mRNA splicing. Mol Biol Cell 29:1111-1124 (2018). PubMed: 29496966