Overview

  • Product name
  • Description
    Rabbit polyclonal to PRP19
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 276-504 of Human PRP19 (Uniprot ID Q9UMS4).

  • Positive control
    • H1299 and Molt-4 whole cell lysates; Raji, 293T, A431, HepG2, and HeLaS3; Human Cal27 xenograft tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab153957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.
  • Tissue specificity
    Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.
  • Sequence similarities
    Belongs to the WD repeat PRP19 family.
    Contains 1 U-box domain.
    Contains 7 WD repeats.
  • Cellular localization
    Nucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPso4 antibody
    • NMP200 antibody
    • Nuclear matrix protein 200 antibody
    • Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody
    • pre mRNA processing factor 19 antibody
    • Pre-mRNA-processing factor 19 antibody
    • PRP19 antibody
    • PRP19/PSO4 homolog antibody
    • PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody
    • PRP19_HUMAN antibody
    • PRPF19 antibody
    • PSO4 antibody
    • psoralen 4 antibody
    • Senescence evasion factor antibody
    • SNEV antibody
    • UBOX4 antibody
    see all

Images

  • All lanes : Anti-PRP19 antibody (ab153957) at 1/3000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Molt-4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling PRP19 with ab153957 at 1/500 dilution. Blue: Costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, labeling PRP19 with ab153957 1/500 dilution.

References

ab153957 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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