Product nameAnti-PRP19 antibody
See all PRP19 primary antibodies
DescriptionRabbit polyclonal to PRP19
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig
Recombinant fragment, corresponding to a region within amino acids 276-504 of Human PRP19 (Uniprot ID Q9UMS4).
- H1299 and Molt-4 whole cell lysates; Raji, 293T, A431, HepG2, and HeLaS3; Human Cal27 xenograft tissue; HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 55 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionPlays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.
Tissue specificityUbiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.
Sequence similaritiesBelongs to the WD repeat PRP19 family.
Contains 1 U-box domain.
Contains 7 WD repeats.
Cellular localizationNucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.
- Information by UniProt
- hPso4 antibody
- NMP200 antibody
- Nuclear matrix protein 200 antibody
All lanes : Anti-PRP19 antibody (ab153957) at 1/3000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : Molt-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling PRP19 with ab153957 at 1/500 dilution. Blue: Costained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, labeling PRP19 with ab153957 1/500 dilution.
ab153957 has not yet been referenced specifically in any publications.