Overview

  • Product name
  • Description
    Rabbit polyclonal to PRP19
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human PRP19 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UMS4

  • Positive control
    • WB: HepG2, NCI-H1299 and HeLa whole cell lysates. Mouse stomach whole cell lysate. ICC/IF: HeLa cells IHC/P: Human cervical cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.
  • Tissue specificity
    Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.
  • Sequence similarities
    Belongs to the WD repeat PRP19 family.
    Contains 1 U-box domain.
    Contains 7 WD repeats.
  • Cellular localization
    Nucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPso4 antibody
    • NMP200 antibody
    • Nuclear matrix protein 200 antibody
    • Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody
    • pre mRNA processing factor 19 antibody
    • Pre-mRNA-processing factor 19 antibody
    • PRP19 antibody
    • PRP19/PSO4 homolog antibody
    • PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody
    • PRP19_HUMAN antibody
    • PRPF19 antibody
    • PSO4 antibody
    • psoralen 4 antibody
    • Senescence evasion factor antibody
    • SNEV antibody
    • UBOX4 antibody
    see all

Images

  • Paraffin-embedded human cervical cancer tissue stained for PRP19 protein using ab228694 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for PRP19 (green) using ab228694 at a 1/200 dilution in ICC/IF.

    Merged with DNA probe (blue).

  • Anti-PRP19 antibody (ab228694) at 1/1000 dilution + Mouse stomach whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-PRP19 antibody (ab228694) at 1/1000 dilution

    Lane 1 : NCI-H1299 (human non-small cell lung carcinoma cell line) whole cell lysates
    Lane 2 : HeLa(Human epithelial cell line from cervix adenocarcinoma) whole cell lysates
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysates

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 55 kDa



    7.5% SDS-PAGE gel.

References

ab228694 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228694.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up