Anti-PRP19 antibody (ab27692)
- Datasheet
- References (10)
- Protocols
Overview
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Product nameAnti-PRP19 antibody
See all PRP19 primary antibodies -
DescriptionRabbit polyclonal to PRP19
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human PRP19.
Read Abcam's proprietary immunogen policy (Peptide available as ab28710.) -
Positive control
- HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate A431 (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab27692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). | |
| IP | Use at an assay dependent concentration. PubMed: 20599946 | |
| ICC/IF | 1/500. |
Target
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FunctionPlays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.
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Tissue specificityUbiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.
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Sequence similaritiesBelongs to the WD repeat PRP19 family.
Contains 1 U-box domain.
Contains 7 WD repeats. -
Cellular localizationNucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.
- Information by UniProt
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Database links
- Entrez Gene: 430767 Chicken
- Entrez Gene: 27339 Human
- Entrez Gene: 28000 Mouse
- Entrez Gene: 246216 Rat
- Omim: 608330 Human
- SwissProt: Q5ZMA2 Chicken
- SwissProt: Q9UMS4 Human
- SwissProt: Q99KP6 Mouse
see all -
Alternative names
- hPso4 antibody
- NMP200 antibody
- Nuclear matrix protein 200 antibody
see all
Images
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Western blot - Anti-PRP19 antibody (ab27692)All lanes : Anti-PRP19 antibody (ab27692) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-PRP19 antibody (ab27692)This image is part of an abreview submitted by Dr Kirk McManusAb27692, at 1/500 dilution, staining human PRP 19 in HeLa Cells by ICC/IF. -
Western blot - Anti-PRP19 antibody (ab27692)All lanes : Anti-PRP19 antibody (ab27692) at 1 µg/ml
Lane 1 :NIH 3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody (ab27692)Image courtesy of Human Protein Atlas. ab27692 staining PRP19 in human breast cancer, showing a distinct nuclear staining pattern. Paraffin embedded human breast cancer tissue was incubated with ab27692 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab27692 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
Protocols
Datasheets and documents
References
This product has been referenced in:
- Hildebrandt A et al. Interaction profiling of RNA-binding ubiquitin ligases reveals a link between posttranscriptional regulation and the ubiquitin system. Sci Rep 7:16582 (2017). Read more (PubMed: 29185492) »
- Dubois JC et al. A phosphorylation-and-ubiquitylation circuitry driving ATR activation and homologous recombination. Nucleic Acids Res 45:8859-8872 (2017). Read more (PubMed: 28666352) »
