Product nameAnti-PRP19 antibody [EPR7446]
See all PRP19 primary antibodies
DescriptionRabbit monoclonal [EPR7446] to PRP19
Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PRP19 aa 450-550. The exact sequence is proprietary.
- Human breast carcinoma and colon carcinoma tissues; HepG2, A431, MCF7, PC12, NIH 3T3, HeLa, K562, and U937 cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 1.06 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- HeLa nuclear extract lysate (ab14655)
- HepG2 cytoplasmic extract lysate (ab14659)
- HepG2 nuclear extract lysate (ab14660)
- K562 nuclear extract lysate (ab14851)
- MCF7 nuclear extract lysate (ab14860)
- NIH 3T3 cytoplasmic extract lysate (ab14873)
- NIH 3T3 nuclear extract lysate (ab14874)
- PC12 cytoplasmic extract lysate (ab14883)
- PC12 nuclear extract lysate (ab14884)
- U937 cytoplasmic extract lysate (ab14904)
- U937 nuclear extract lysate (TPA stimulated) (ab14906)
Our Abpromise guarantee covers the use of ab126776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/250 - 1/500.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPlays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.
Tissue specificityUbiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.
Sequence similaritiesBelongs to the WD repeat PRP19 family.
Contains 1 U-box domain.
Contains 7 WD repeats.
Cellular localizationNucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.
- Information by UniProt
- hPso4 antibody
- NMP200 antibody
- Nuclear matrix protein 200 antibody
All lanes : Anti-PRP19 antibody [EPR7446] (ab126776) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : PC12 cell lysate
Lane 5 : NIH 3T3 cell lysate
Lane 6 : HeLa cell lysate
Lane 7 : K562 cell lysate
Lane 8 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 55 kDa
ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
Equilibrium disassociation constant (KD)
Learn more about KD
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