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RecombinantRabMAb

Recombinant Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)

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Western blot - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
  • OI-RD Scanning - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
  • Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7446] to PRP19 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-PRP19 antibody [EPR7446] - BSA and Azide free
    See all PRP19 primary antibodies

  • Description

    Rabbit monoclonal [EPR7446] to PRP19 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248181 is the carrier-free version of ab126776.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Application notes
Is unsuitable for IP.

Target

  • Function

    Plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.

  • Tissue specificity

    Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.

  • Sequence similarities

    Belongs to the WD repeat PRP19 family.
    Contains 1 U-box domain.
    Contains 7 WD repeats.

  • Cellular localization

    Nucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • hPso4 antibody
    • NMP200 antibody
    • Nuclear matrix protein 200 antibody
    • Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody
    • pre mRNA processing factor 19 antibody
    • Pre-mRNA-processing factor 19 antibody
    • PRP19 antibody
    • PRP19/PSO4 homolog antibody
    • PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody
    • PRP19_HUMAN antibody
    • PRPF19 antibody
    • PSO4 antibody
    • psoralen 4 antibody
    • Senescence evasion factor antibody
    • SNEV antibody
    • UBOX4 antibody
    see all

Images

  • Western blot - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
    Western blot - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
    All lanes : Anti-PRP19 antibody [EPR7446] (ab126776) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : MCF-7 cell lysate
    Lane 4 : PC-12 cell lysate
    Lane 5 : NIH/3T3 cell lysate
    Lane 6 : HeLa cell lysate
    Lane 7 : K562 cell lysate
    Lane 8 : U937 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 55 kDa



    This data was developed using ab126776, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
    This data was developed using ab126776, the same antibody clone in a different buffer formulation.ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
    This data was developed using ab126776, the same antibody clone in a different buffer formulation.ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
    OI-RD Scanning - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
    This data was developed using ab126776, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
    Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab248181? Please let us know so that we can cite the reference in this datasheet.

ab248181 has not yet been referenced specifically in any publications.

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