Recombinant
RabMAb

Recombinant Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7446] to PRP19 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PRP19 antibody [EPR7446] - BSA and Azide free
    See all PRP19 primary antibodies

  • Description

    Rabbit monoclonal [EPR7446] to PRP19 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PRP19 aa 450-550. The exact sequence is proprietary.

  • General notes

    Ab248181 is the carrier-free version of ab126776. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248181 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab248181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.

    • Tissue specificity

      Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.

    • Sequence similarities

      Belongs to the WD repeat PRP19 family.
      Contains 1 U-box domain.
      Contains 7 WD repeats.

    • Cellular localization

      Nucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • hPso4 antibody
      • NMP200 antibody
      • Nuclear matrix protein 200 antibody
      • Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody
      • pre mRNA processing factor 19 antibody
      • Pre-mRNA-processing factor 19 antibody
      • PRP19 antibody
      • PRP19/PSO4 homolog antibody
      • PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody
      • PRP19_HUMAN antibody
      • PRPF19 antibody
      • PSO4 antibody
      • psoralen 4 antibody
      • Senescence evasion factor antibody
      • SNEV antibody
      • UBOX4 antibody
      see all

    Images

    • Western blot - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
      Western blot - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
      All lanes : Anti-PRP19 antibody [EPR7446] (ab126776) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : MCF-7 cell lysate
      Lane 4 : PC-12 cell lysate
      Lane 5 : NIH/3T3 cell lysate
      Lane 6 : HeLa cell lysate
      Lane 7 : K562 cell lysate
      Lane 8 : U937 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

      Predicted band size: 55 kDa



      This data was developed using ab126776, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
      This data was developed using ab126776, the same antibody clone in a different buffer formulation.

      ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
      This data was developed using ab126776, the same antibody clone in a different buffer formulation.

      ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Other - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
      Other - Anti-PRP19 antibody [EPR7446] - BSA and Azide free (ab248181)
      This data was developed using ab126776, the same antibody clone in a different buffer formulation.

      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab248181? Please let us know so that we can cite the reference in this datasheet.

    ab248181 has not yet been referenced specifically in any publications.

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