Overview

  • Product name

  • Description

    Rabbit polyclonal to PRP6/ANT-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human PRP6/ANT-1 aa 1-50.
    Database link: NP_036601.2

  • Positive control

    • HeLa, 293T and NIH3T3 whole cell lysate
  • General notes

     This product was previously labelled as PRP6

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab99292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 107 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in pre-mRNA splicing. May act in the tri-snRNP complex as a bridging factor between U5 and U4/U6 snRNPs in the late step of spliceosome assembly. May be necessary for tri-snRNP formation.
  • Sequence similarities

    Contains 9 HAT repeats.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Androgen receptor N terminal domain transactivating protein 1 antibody
    • ANT 1 antibody
    • ANT1 antibody
    • C20orf14 antibody
    • hPrp6 antibody
    • p102 U5 small nuclear ribonucleoprotein particle binding protein antibody
    • pre-mRNA processing factor 6 antibody
    • Pre-mRNA-processing factor 6 antibody
    • PRP6 homolog antibody
    • PRP6 pre mRNA processing factor 6 homolog antibody
    • PRP6_HUMAN antibody
    • PRPF6 antibody
    • Putative mitochondrial outer membrane protein import receptor antibody
    • TOM antibody
    • U5 snRNP-associated 102 kDa protein antibody
    • U5-102 kDa protein antibody
    • U5-102K antibody
    see all

Images

  • All lanes : Anti-PRP6/ANT-1 antibody (ab99292) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 107 kDa


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PRP6/ANT-1 with ab99292 at 1/200 (1µg/ml). Detection: DAB.

  • Lane 1 : Detection of Human PRP6/ANT-1 by immunoprecipitation from HeLa (1 mg for IP, 20% of IP loaded), using 3µg/ml ab99292. Lane 2: Control IgG

References

This product has been referenced in:

  • Cloutier P  et al. R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun 8:15615 (2017). WB . Read more (PubMed: 28561026) »
  • Liu YC  et al. Cytoplasmic Viral RNA-Dependent RNA Polymerase Disrupts the Intracellular Splicing Machinery by Entering the Nucleus and Interfering with Prp8. PLoS Pathog 10:e1004199 (2014). WB ; Human . Read more (PubMed: 24968230) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab99292.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up