Product nameAnti-PRPF31 antibody
See all PRPF31 primary antibodies
DescriptionGoat polyclonal to PRPF31
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
- HeLa cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab58588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. Also a band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in pre-mRNA splicing. Required for U4/U6.U5 tri-snRNP formation.
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in PRPF31 are the cause of retinitis pigmentosa type 11 (RP11) [MIM:600138]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP11 inheritance is autosomal dominant.
Sequence similaritiesBelongs to the PRP31 family.
Contains 1 Nop domain.
DomainInteracts with the snRNP via the Nop domain.
The coiled coil domain is formed by two non-contiguous helices.
Cellular localizationNucleus speckle. Nucleus > Cajal body. Predominantly found in speckles and in Cajal bodies.
- Information by UniProt
- DKFZp566J153 antibody
- hPrp 31 antibody
- hPrp31 antibody
All lanes : Anti-PRPF31 antibody (ab58588) at 1 µg/ml
Lane 1 : HeLa cell lysate (35µg protein in RIPA buffer) without blocking with the immunizing peptide
Lane 2 : HeLa cell lysate (35µg protein in RIPA buffer) with blocking with the immunizing peptide
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence.
ab58588 has not yet been referenced specifically in any publications.