Overview

  • Product name

  • Description

    Rabbit polyclonal to PRPF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PRPF4 aa 1-260.
    Database link: O43172

  • Positive control

    • WB: Jurkat, HeLa and HepG2 whole cell lysates. IHC-P: Human ovarian cancer and kidney tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab229887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

Target

  • Function

    Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
  • Sequence similarities

    Contains 7 WD repeats.
  • Cellular localization

    Nucleus speckle. Colocalizes with spliceosomal snRNPs.
  • Information by UniProt
  • Database links

  • Alternative names

    • hPrp4 antibody
    • HPRP4P antibody
    • PRP4 antibody
    • PRP4 homolog antibody
    • PRP4 pre mRNA processing factor 4 homolog (yeast) antibody
    • PRP4/STK/WD splicing factor antibody
    • PRP4_HUMAN antibody
    • Prp4p antibody
    • PRPF4 antibody
    • RP70 antibody
    • SNRNP60 antibody
    • U4/U6 small nuclear ribonucleoprotein Prp4 antibody
    • U4/U6 snRNP 60 kDa protein antibody
    • WD splicing factor Prp4 antibody
    see all

Images

  • All lanes : Anti-PRPF4 antibody (ab229887) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • Paraffin-embedded human ovarian cancer tissue stained for PRPF4 using ab229887 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human kidney tissue stained for PRPF4 using ab229887 at 1/100 dilution in immunohistochemical analysis.

References

ab229887 has not yet been referenced specifically in any publications.

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