Overview

  • Product name

    Anti-PRPF40B antibody
  • Description

    Rabbit polyclonal to PRPF40B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: KSNSPESETD PEEKAGKESD EKEQEQDKDR ELQQAELPNR SPGFGIKKEK TGWDTSESE, corresponding to C terminal amino acids 792-850 of Human PRPF40B.

  • Positive control

    • Human cerebellum tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250. Predicted molecular weight: 99 kDa.

Target

  • Function

    May be involved in pre-mRNA splicing.
  • Tissue specificity

    Expressed in the striatum and cortex of the brain (at protein level). Highly expressed in testis, fetal kidney and fetal brain. Moderately expressed in pancreas, skeletal muscle, placenta, brain and heart. Weakly expressed in colon, ileum, ovary, prostate, spleen, kidney and fetal lung.
  • Sequence similarities

    Belongs to the PRPF40 family.
    Contains 2 FF domains.
    Contains 2 WW domains.
  • Cellular localization

    Nucleus speckle.
  • Information by UniProt
  • Database links

  • Alternative names

    • Huntingtin interacting protein C antibody
    • Huntingtin yeast partner C antibody
    • Huntingtin-interacting protein C antibody
    • HYPC antibody
    • PR40B_HUMAN antibody
    • pre mRNA processing factor 40 homolog B antibody
    • Pre-mRNA-processing factor 40 homolog B antibody
    • PRP40 pre mRNA processing factor 40 homolog B antibody
    • PRPF40B antibody
    see all

Images

  • All lanes : Anti-PRPF40B antibody (ab122474) at 1/100 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver
    Lane 5 : Human tonsil

    Predicted band size: 99 kDa

  • ab122474, at 1/200 dilution, staining PRPF40B in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.

References

ab122474 has not yet been referenced specifically in any publications.

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