Overview

  • Product name
  • Description
    Rabbit polyclonal to PRPF8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 850-900 of Human PRPF8 according to NP_006436.3.

  • Positive control
    • 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 274 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes.
    • Tissue specificity
      Widely expressed.
    • Involvement in disease
      Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:600059]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.
    • Sequence similarities
      Contains 1 MPN (JAB/Mov34) domain.
    • Domain
      The MPN domain has structural similarity with viral ribonucleases and RNase H, but unlike RNases, it does not bind any metal ions.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus speckle.
    • Information by UniProt
    • Database links
    • Alternative names
      • 220 kDa U5 snRNP specific protein antibody
      • 220 kDa U5 snRNP-specific protein antibody
      • Apoptosis regulated protein 1 antibody
      • Apoptosis regulated protein 2 antibody
      • HPRP8 antibody
      • p220 antibody
      • Pre mRNA processing factor 8 antibody
      • Pre mRNA-processing factor 8, S. cerevisiae, homolog of antibody
      • Pre-mRNA-processing-splicing factor 8 antibody
      • Precursor mRNA processing protein antibody
      • PRP8 antibody
      • PRP8 homolog antibody
      • PRP8 pre mRNA processing factor 8 homolog antibody
      • PRP8_HUMAN antibody
      • PRPC8 antibody
      • Prpf8 antibody
      • Retinitis pigmentosa 13 (autosomal dominant) antibody
      • RP13 antibody
      • SNRNP220 antibody
      • Splicing factor Prp8 antibody
      • U5 snRNP specific protein antibody
      • U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p antibody
      see all

    Images

    • All lanes : Anti-PRPF8 antibody (ab157114) at 0.1 µg/ml

      Lane 1 : 293T whole cell lysate at 50 µg
      Lane 2 : 293T whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg
      Lane 5 : NIH 3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 274 kDa


      Exposure time: 3 seconds

    References

    ab157114 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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