Recombinant
RabMAb

Anti-PRPF8 antibody [EPR15228] (ab190347)

Overview

  • Product name
    Anti-PRPF8 antibody [EPR15228]
    See all PRPF8 primary antibodies
  • Description
    Rabbit monoclonal [EPR15228] to PRPF8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PRPF8 aa 200-300. The exact sequence is proprietary.
    Database link: Q6P2Q9

  • Positive control
    • HeLa, HepG2, K562, 293T, mouse RAW 264.7 and rat PC12 cell lysates. Human ovarian carcinoma and rat spleen tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 274 kDa (predicted molecular weight: 274 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes.
  • Tissue specificity
    Widely expressed.
  • Involvement in disease
    Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:600059]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.
  • Sequence similarities
    Contains 1 MPN (JAB/Mov34) domain.
  • Domain
    The MPN domain has structural similarity with viral ribonucleases and RNase H, but unlike RNases, it does not bind any metal ions.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • 220 kDa U5 snRNP specific protein antibody
    • 220 kDa U5 snRNP-specific protein antibody
    • Apoptosis regulated protein 1 antibody
    • Apoptosis regulated protein 2 antibody
    • HPRP8 antibody
    • p220 antibody
    • Pre mRNA processing factor 8 antibody
    • Pre mRNA-processing factor 8, S. cerevisiae, homolog of antibody
    • Pre-mRNA-processing-splicing factor 8 antibody
    • Precursor mRNA processing protein antibody
    • PRP8 antibody
    • PRP8 homolog antibody
    • PRP8 pre mRNA processing factor 8 homolog antibody
    • PRP8_HUMAN antibody
    • PRPC8 antibody
    • Prpf8 antibody
    • Retinitis pigmentosa 13 (autosomal dominant) antibody
    • RP13 antibody
    • SNRNP220 antibody
    • Splicing factor Prp8 antibody
    • U5 snRNP specific protein antibody
    • U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p antibody
    see all

Images

  • All lanes : Anti-PRPF8 antibody [EPR15228] (ab190347) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 274 kDa
    Observed band size: 274 kDa

  • Anti-PRPF8 antibody [EPR15228] (ab190347) at 1/20000 dilution + 293T cell lysate at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 274 kDa
    Observed band size: 274 kDa

  • All lanes : Anti-PRPF8 antibody [EPR15228] (ab190347) at 1/1000 dilution

    Lane 1 : mouse RAW 264.7 cell lysate
    Lane 2 : rat PC12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 274 kDa
    Observed band size: 274 kDa

  • Immunohistochemical analysis of paraffin-embedded, Human ovarian carcinoma tissue labeling PRPF8 with ab190347 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

  • Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling PRPF8 with ab190347 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

References

ab190347 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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