Overview

  • Product name

  • Description

    Rabbit polyclonal to PRPF8/Prp8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human PRPF8/Prp8 aa 1978-2072 (C terminal).
    Database link: Q6P2Q9

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji cells.
  • General notes

    Previously labelled as PRPF8. 

Properties

Applications

Our Abpromise guarantee covers the use of ab137694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 274 kDa.

Target

  • Function

    Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes.
  • Tissue specificity

    Widely expressed.
  • Involvement in disease

    Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:600059]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.
  • Sequence similarities

    Contains 1 MPN (JAB/Mov34) domain.
  • Domain

    The MPN domain has structural similarity with viral ribonucleases and RNase H, but unlike RNases, it does not bind any metal ions.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus speckle.
  • Information by UniProt
  • Database links

  • Alternative names

    • 220 kDa U5 snRNP specific protein antibody
    • 220 kDa U5 snRNP-specific protein antibody
    • Apoptosis regulated protein 1 antibody
    • Apoptosis regulated protein 2 antibody
    • HPRP8 antibody
    • p220 antibody
    • Pre mRNA processing factor 8 antibody
    • Pre mRNA-processing factor 8, S. cerevisiae, homolog of antibody
    • Pre-mRNA-processing-splicing factor 8 antibody
    • Precursor mRNA processing protein antibody
    • PRP8 antibody
    • PRP8 homolog antibody
    • PRP8 pre mRNA processing factor 8 homolog antibody
    • PRP8_HUMAN antibody
    • PRPC8 antibody
    • Prpf8 antibody
    • Retinitis pigmentosa 13 (autosomal dominant) antibody
    • RP13 antibody
    • SNRNP220 antibody
    • Splicing factor Prp8 antibody
    • U5 snRNP specific protein antibody
    • U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p antibody
    see all

Images

  • Anti-PRPF8/Prp8 antibody (ab137694) at 1/500 dilution + whole cell lysate prepared from Raji cells at 30 µg

    Predicted band size: 274 kDa



    5% SDS PAGE

References

This product has been referenced in:

  • Mir RA  et al. A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression. Mol Cell Biol 36:886-99 (2015). Read more (PubMed: 26711270) »
See 1 Publication for this product

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