Anti-PRPF8/Prp8 antibody (ab245504)

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Overview

  • Product name

    Anti-PRPF8/Prp8 antibody
    See all PRPF8/Prp8 primary antibodies

  • Description

    Rabbit polyclonal to PRPF8/Prp8

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human PRPF8/Prp8 aa 1825-1875. The exact sequence is proprietary. (NP_006436.3).
    Database link: Q6P2Q9

  • Positive control

    • WB: HEK-293T, HeLa, Jurkat and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab245504 was affinity purified using an epitope specific to PRPF8/Prp8 immobilized on solid support.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 274 kDa.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes.

  • Tissue specificity

    Widely expressed.

  • Involvement in disease

    Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:600059]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.

  • Sequence similarities

    Contains 1 MPN (JAB/Mov34) domain.

  • Domain

    The MPN domain has structural similarity with viral ribonucleases and RNase H, but unlike RNases, it does not bind any metal ions.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus speckle.
  • Information by UniProt

  • Database links

    • Alternative names

      • 220 kDa U5 snRNP specific protein antibody
      • 220 kDa U5 snRNP-specific protein antibody
      • Apoptosis regulated protein 1 antibody
      • Apoptosis regulated protein 2 antibody
      • HPRP8 antibody
      • p220 antibody
      • Pre mRNA processing factor 8 antibody
      • Pre mRNA-processing factor 8, S. cerevisiae, homolog of antibody
      • Pre-mRNA-processing-splicing factor 8 antibody
      • Precursor mRNA processing protein antibody
      • PRP8 antibody
      • PRP8 homolog antibody
      • PRP8 pre mRNA processing factor 8 homolog antibody
      • PRP8_HUMAN antibody
      • PRPC8 antibody
      • Prpf8 antibody
      • Retinitis pigmentosa 13 (autosomal dominant) antibody
      • RP13 antibody
      • SNRNP220 antibody
      • Splicing factor Prp8 antibody
      • U5 snRNP specific protein antibody
      • U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p antibody
      see all

    Images

    • Western blot - Anti-PRPF8/Prp8 antibody (ab245504)
      Western blot - Anti-PRPF8/Prp8 antibody (ab245504)
      All lanes : Anti-PRPF8/Prp8 antibody (ab245504) at 0.1 µg/ml

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
      Lane 2 : HEK-293T whole cell lysate at 15 µg
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
      Lane 5 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 274 kDa


      Exposure time: 10 seconds
    • Immunoprecipitation - Anti-PRPF8/Prp8 antibody (ab245504)
      Immunoprecipitation - Anti-PRPF8/Prp8 antibody (ab245504)

      PRPF8/Prp8 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245504 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245504 at 1 µg/ml.

      Lane 1: ab245504 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody (ab245504)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody (ab245504)

      Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PRPF8/Prp8 using ab245504 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

    References

    ab245504 has not yet been referenced specifically in any publications.

    Publishing research using ab245504? Please let us know so that we can cite the reference in this datasheet.

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