Product nameAnti-PRPF8/Prp8 antibody
See all PRPF8/Prp8 primary antibodies
DescriptionRabbit polyclonal to PRPF8/Prp8
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human PRPF8/Prp8 aa 1825-1875. The exact sequence is proprietary. (NP_006436.3).
Database link: Q6P2Q9
- WB: HEK-293T, HeLa, Jurkat and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245504 was affinity purified using an epitope specific to PRPF8/Prp8 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 274 kDa.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCentral component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes.
Tissue specificityWidely expressed.
Involvement in diseaseDefects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:600059]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.
Sequence similaritiesContains 1 MPN (JAB/Mov34) domain.
DomainThe MPN domain has structural similarity with viral ribonucleases and RNase H, but unlike RNases, it does not bind any metal ions.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus speckle.
- Information by UniProt
- 220 kDa U5 snRNP specific protein antibody
- 220 kDa U5 snRNP-specific protein antibody
- Apoptosis regulated protein 1 antibody
All lanes : Anti-PRPF8/Prp8 antibody (ab245504) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 274 kDa
Exposure time: 10 seconds
PRPF8/Prp8 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245504 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245504 at 1 µg/ml.
Lane 1: ab245504 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PRPF8/Prp8 using ab245504 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
ab245504 has not yet been referenced specifically in any publications.