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    prps1-antibody-ab137577.pdf

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Anti-PRPS1 antibody (ab137577)

  • Datasheet
  • SDS
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Western blot - Anti-PRPS1 antibody (ab137577)
  • Immunocytochemistry/ Immunofluorescence - Anti-PRPS1 antibody (ab137577)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPS1 antibody (ab137577)

Key features and details

  • Rabbit polyclonal to PRPS1
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PRPS1 antibody
    See all PRPS1 primary antibodies
  • Description

    Rabbit polyclonal to PRPS1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 142-307 of Human PRPS1.

  • Positive control

    • 293T, H1299, HeLa, HepG2, Molt 4 and Raji cell lysates; HeLa cells; SW480 xenograft

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human PRPS1 protein (ab92935)
  • Related Products

    • Recombinant Human PRPS1 protein (ab92935)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Cow
Xenopus laevis
Xenopus tropicalis
Application Abreviews Notes
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
Notes
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa.

Target

  • Function

    Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.
  • Pathway

    Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.
  • Involvement in disease

    Defects in PRPS1 are the cause of phosphoribosylpyrophosphate synthetase superactivity (PRPS1 superactivity) [MIM:300661]; also known as PRPS-related gout. It is a familial disorder characterized by excessive purine production, gout and uric acid urolithiasis.
    Defects in PRPS1 are the cause of Charcot-Marie-Tooth disease X-linked recessive type 5 (CMTX5) [MIM:311070]; also known as optic atrophy-polyneuropathy-deafness or Rosenberg-Chutorian syndrome. CMTX5 is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy.
    Defects in PRPS1 are the cause of ARTS syndrome (ARTS) [MIM:301835]; also known as fatal ataxia X-linked with deafness and loss of vision. ARTS is a disorder characterized by mental retardation, early-onset hypotonia, ataxia, delayed motor development, hearing impairment, and optic atrophy. Susceptibility to infections, especially of the upper respiratory tract, can result in early death.
    Defects in PRPS1 are the cause of deafness X-linked type 1 (DFNX1) [MIM:304500]; also known as congenital sensorineural deafness X-linked 2 (DFN2). It is a form of deafness characterized by progressive, severe-to-profound sensorineural hearing loss in males. Females manifest mild to moderate hearing loss.
  • Sequence similarities

    Belongs to the ribose-phosphate pyrophosphokinase family.
  • Target information above from: UniProt accession P60891 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 781227 Cow
    • Entrez Gene: 5631 Human
    • Entrez Gene: 19139 Mouse
    • Entrez Gene: 29562 Rat
    • Entrez Gene: 314140 Rat
    • Omim: 311850 Human
    • SwissProt: Q2HJ58 Cow
    • SwissProt: P60891 Human
    • SwissProt: Q9D7G0 Mouse
    • SwissProt: P60892 Rat
    • Unigene: 56 Human
    • Unigene: 287178 Mouse
    • Unigene: 91470 Rat
    • Unigene: 9761 Rat
    see all
  • Alternative names

    • ARTS antibody
    • CMTX5 antibody
    • Deafness 2 perceptive congenital antibody
    • Deafness X linked 2 perceptive congenital antibody
    • DFN2 antibody
    • DFNX1 antibody
    • EC 2.7.6.1 antibody
    • KIAA0967 antibody
    • Phosphoribosyl pyrophosphate synthase I antibody
    • Phosphoribosyl pyrophosphate synthetase I antibody
    • PPRibP antibody
    • Prps1 antibody
    • PRPS1_HUMAN antibody
    • PRS I antibody
    • PRS-I antibody
    • PRSI antibody
    • Ribose phosphate pyrophosphokinase I antibody
    • Ribose-phosphate pyrophosphokinase 1 antibody
    see all

Images

  • Western blot - Anti-PRPS1 antibody (ab137577)
    Western blot - Anti-PRPS1 antibody (ab137577)
    Anti-PRPS1 antibody (ab137577) at 1/1000 dilution + Molt 4 whole cell lysate at 30 µg

    Predicted band size: 34 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-PRPS1 antibody (ab137577)
    Immunocytochemistry/ Immunofluorescence - Anti-PRPS1 antibody (ab137577)
    Immunofluorescent analysis of methanol-fixed HeLa cells labelling PRPS1 with ab137577 at 1/200 dilution. Lower image is costained with Hoechst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPS1 antibody (ab137577)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPS1 antibody (ab137577)
    Immunohistochemical analysis of paraffin-embedded SW480 xenograft labelling PRPS1 with ab137577 at 1/500 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab137577? Please let us know so that we can cite the reference in this datasheet.

    ab137577 has been referenced in 1 publication.

    • Ma Y  et al. High expression of PRPS1 induces an anti-apoptotic effect in B-ALL cell lines and predicts an adverse prognosis in Chinese children with B-ALL. Oncol Lett 15:4314-4322 (2018). PubMed: 29541198

    Customer reviews and Q&As

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