Key features and details
- Rabbit polyclonal to PRPS2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRPS2 antibody
See all PRPS2 primary antibodies
DescriptionRabbit polyclonal to PRPS2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish
Recombinant fragment containing a sequence corresponding to a region within amino acids 1-253 of Human PRPS2 (NP_001034180).
- HepG2 whole cell lysate; HeLa cells; Hep3B; 293T, A431, H1299, HeLaS3, MOLT4 and Raji cell lines
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 35 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/100 - 1/200.|
FunctionCatalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.
PathwayMetabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.
Sequence similaritiesBelongs to the ribose-phosphate pyrophosphokinase family.
- Information by UniProt
- EC 126.96.36.199 antibody
- Phosphoribosyl pyrophosphate synthase II antibody
- phosphoribosyl pyrophosphate synthetase 2 antibody
Anti-PRPS2 antibody (ab96222) at 1/1000 dilution + HepG2 whole cell lysate
at 30 µg
Predicted band size: 35 kDa
10% SDS PAGE
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using ab96222 at 1/200 dilution: (1) without DNA probe; (2) merged with a DNA probe.
Immunohistochemical analysis of paraffin-embedded Hep3B using ab96222 at 1/100 dilution.
ab96222 has not yet been referenced specifically in any publications.