Anti-PRRG2 antibody (ab228870)
Key features and details
- Rabbit polyclonal to PRRG2
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PRRG2 antibody -
Description
Rabbit polyclonal to PRRG2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human PRRG2 (internal sequence). The exact sequence is proprietary.
Database link: O14669 -
Positive control
- WB: NCI-H1299, HCT 116 and A549 whole cell extract; A549 membrane extract. IHC-P: Rat thyroid gland tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab228870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 22 kDa. |
Target
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Relevance
PRRG2 interacts with NEDD4 and is highly expressed in the thyroid. -
Cellular localization
Cell Membrane; Single-pass type I membrane protein. -
Database links
- Entrez Gene: 5639 Human
- Entrez Gene: 361570 Rat
- Omim: 604429 Human
- SwissProt: O14669 Human
- Unigene: 35101 Human
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Alternative names
- PRGP2 antibody
- Proline rich gamma carboxyglutamic acid protein 2 antibody
- Proline rich Gla (G carboxyglutamic acid) polypeptide 2 antibody
see all
Images
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Western blot - Anti-PRRG2 antibody (ab228870)All lanes : Anti-PRRG2 antibody (ab228870) at 1/1000 dilution
Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell extract
Lane 3 : A549 (human lung carcinoma cell line) whole cell extract
Lane 4 : A549 membrane extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa15% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRRG2 antibody (ab228870)Paraffin-embedded rat thyroid gland tissue stained for PRRG2 with ab228870 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab228870 has not yet been referenced specifically in any publications.
